PMID- 21344225
OWN - NLM
STAT- MEDLINE
DCOM- 20111205
LR  - 20211020
IS  - 1432-0584 (Electronic)
IS  - 0939-5555 (Print)
IS  - 0939-5555 (Linking)
VI  - 90
IP  - 11
DP  - 2011 Nov
TI  - Nuclei micro-array FISH, a desirable alternative for MCL diagnosis.
PG  - 1299-305
LID - 10.1007/s00277-011-1197-0 [doi]
AB  - Mantle cell lymphoma (MCL) is a rare, specific lymphoma subtype. Though the 
      morphologic and immunophenotypic features of MCL have been well described in 
      recent literatures, it is still a diagnostic dilemma because of its frequent 
      confusion with other small B cell lymphomas (SBCLs). In the present study, we 
      primarily focus on establishing a sensitive and specific method for the diagnosis 
      of MCL, which is efficient to distinguish this disease from other SBCLs. We 
      carried out our investigation for MCL and other SBCLs (including SLL, FL, MZL, 
      and MALT) on their feature of morphology, immunophenotype, and t(11;14)(q13;q32) 
      translocation analysis based on polymerase chain reaction (PCR) and interphase 
      nuclei micro-array fluorescence in situ hybridization (FISH). The morphologic and 
      immunologic analysis showed the positive rate of cyclin D1 was 76.47% in MCL, 
      which was significantly higher than that in other SBCLs. The positive rate of 
      t(11;14) translocation was 25.81% and 35.48%, respectively, tested by general and 
      semi-nested PCR, while 93.10% positive rate was shown with low background and 
      strong signals pattern when tested by Nuclei micro-array FISH. Our research shows 
      that t(11;14) translocation is a special and useful diagnostic marker for MCL, 
      and detection of the marker by nuclei micro-array FISH is convenient and 
      economic, especially more sensitive and specific than other methods for the 
      diagnosis of MCL.
FAU - Chu, Hongjuan
AU  - Chu H
AD  - Department of Pathology, Southern Hospital, Southern Medical University, 
      Guangzhou 510515, Guangdong Province, People's Republic of China.
FAU - Han, Xiqun
AU  - Han X
FAU - Jiang, Huiyong
AU  - Jiang H
FAU - Li, Feng
AU  - Li F
FAU - Li, Huiling
AU  - Li H
FAU - Zhao, Tong
AU  - Zhao T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110223
PL  - Germany
TA  - Ann Hematol
JT  - Annals of hematology
JID - 9107334
RN  - 0 (Biomarkers)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers/metabolism
MH  - Cell Nucleus/*genetics
MH  - Chromosomes, Human, Pair 11
MH  - Female
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphoma, Mantle-Cell/*diagnosis/*genetics/pathology
MH  - Male
MH  - Microarray Analysis/*methods
MH  - Middle Aged
MH  - Polymerase Chain Reaction/methods
MH  - Prognosis
MH  - Sensitivity and Specificity
MH  - Translocation, Genetic
MH  - Young Adult
PMC - PMC3187854
EDAT- 2011/02/24 06:00
MHDA- 2011/12/13 00:00
PMCR- 2011/02/23
CRDT- 2011/02/24 06:00
PHST- 2010/03/01 00:00 [received]
PHST- 2011/02/09 00:00 [accepted]
PHST- 2011/02/24 06:00 [entrez]
PHST- 2011/02/24 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
PHST- 2011/02/23 00:00 [pmc-release]
AID - 1197 [pii]
AID - 10.1007/s00277-011-1197-0 [doi]
PST - ppublish
SO  - Ann Hematol. 2011 Nov;90(11):1299-305. doi: 10.1007/s00277-011-1197-0. Epub 2011 
      Feb 23.