PMID- 21345559
OWN - NLM
STAT- MEDLINE
DCOM- 20110721
LR  - 20191210
IS  - 1873-6424 (Electronic)
IS  - 0269-7491 (Linking)
VI  - 159
IP  - 5
DP  - 2011 May
TI  - A rapid and high-throughput quantum dots bioassay for monitoring of 
      perfluorooctane sulfonate in environmental water samples.
PG  - 1348-53
LID - 10.1016/j.envpol.2011.01.011 [doi]
AB  - Currently HPLC/MS is the state of the art tool for environmental/drinking water 
      perfluorooctane sulfonate (PFOS) monitoring. PFOS can bind to peroxisomal 
      proliferator-activated receptor-alpha (PPARalpha), which forms heterodimers with 
      retinoid X receptors (RXRs) and binds to PPAR response elements. In this bioassay 
      free PFOS in water samples competes with immobilized PFOS in ELISA plates for a 
      given amount of PPARalpha-RXRalpha. It can be determined indirectly by immobilizing 
      PPARalpha-RXRalpha-PFOS complex to another plate coated with PPARalpha antibody and 
      subsequent measuring the level of PPARalpha-RXRalpha by using biotin-modified PPARalpha-RXRalpha 
      probes-quantum dots-streptavidin detection system. The rapid and high-throughput 
      bioassay demonstrated a detection limit of 2.5 ng L(-1) with linear range between 
      2.5 ng L(-1) and 75 ng L(-1). Detection results of environmental water samples 
      were highly consistent between the bioassay and HPLC/MS.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Zhang, Jiong
AU  - Zhang J
AD  - Minister of Education Key Laboratory of Environment and Health, School of Public 
      Health, Tongji Medical College, Huazhong University of Science and Technology, 
      Wuhan, Hubei Province 430030, PR China.
FAU - Wan, Yanjian
AU  - Wan Y
FAU - Li, Yuanyuan
AU  - Li Y
FAU - Zhang, Qiongfang
AU  - Zhang Q
FAU - Xu, Shunqing
AU  - Xu S
FAU - Zhu, Huijun
AU  - Zhu H
FAU - Shu, Baihua
AU  - Shu B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20110222
PL  - England
TA  - Environ Pollut
JT  - Environmental pollution (Barking, Essex : 1987)
JID - 8804476
RN  - 0 (Alkanesulfonic Acids)
RN  - 0 (Fluorocarbons)
RN  - 0 (Indicators and Reagents)
RN  - 0 (PPAR alpha)
RN  - 0 (Retinoid X Receptor alpha)
RN  - 0 (Water Pollutants, Chemical)
RN  - 9013-20-1 (Streptavidin)
RN  - 9H2MAI21CL (perfluorooctane sulfonic acid)
SB  - IM
MH  - Alkanesulfonic Acids/*analysis/chemistry
MH  - Chromatography, High Pressure Liquid
MH  - Environmental Monitoring/*methods
MH  - Fluorocarbons/*analysis/chemistry
MH  - Fresh Water/analysis
MH  - Indicators and Reagents
MH  - Mass Spectrometry
MH  - PPAR alpha
MH  - Quantum Dots
MH  - Retinoid X Receptor alpha
MH  - Streptavidin
MH  - Water Pollutants, Chemical/*analysis
EDAT- 2011/02/25 06:00
MHDA- 2011/07/22 06:00
CRDT- 2011/02/25 06:00
PHST- 2010/09/08 00:00 [received]
PHST- 2010/12/24 00:00 [revised]
PHST- 2011/01/07 00:00 [accepted]
PHST- 2011/02/25 06:00 [entrez]
PHST- 2011/02/25 06:00 [pubmed]
PHST- 2011/07/22 06:00 [medline]
AID - S0269-7491(11)00033-9 [pii]
AID - 10.1016/j.envpol.2011.01.011 [doi]
PST - ppublish
SO  - Environ Pollut. 2011 May;159(5):1348-53. doi: 10.1016/j.envpol.2011.01.011. Epub 
      2011 Feb 22.