PMID- 21345948
OWN - NLM
STAT- MEDLINE
DCOM- 20110614
LR  - 20220129
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 85
IP  - 9
DP  - 2011 May
TI  - Unique spectrum of activity of prosimian TRIM5alpha against exogenous and 
      endogenous retroviruses.
PG  - 4173-83
LID - 10.1128/JVI.00075-11 [doi]
AB  - Lentiviruses, the genus of retrovirus that includes HIV-1, rarely endogenize. 
      Some lemurs uniquely possess an endogenous lentivirus called PSIV ("prosimian 
      immunodeficiency virus"). Thus, lemurs provide the opportunity to study the 
      activity of host defense factors, such as TRIM5alpha, in the setting of germ line 
      invasion. We characterized the activities of TRIM5alpha proteins from two distant 
      lemurs against exogenous retroviruses and a chimeric PSIV. TRIM5alpha from gray mouse 
      lemur, which carries PSIV in its genome, exhibited the narrowest restriction 
      activity. One allelic variant of gray mouse lemur TRIM5alpha restricted only N-tropic 
      murine leukemia virus (N-MLV), while a second variant restricted N-MLV and, 
      uniquely, B-tropic MLV (B-MLV); both variants poorly blocked PSIV. In contrast, 
      TRIM5alpha from ring-tailed lemur, which does not contain PSIV in its genome, 
      revealed one of the broadest antiviral activities reported to date against 
      lentiviruses, including PSIV. Investigation into the antiviral specificity of 
      ring-tailed lemur TRIM5alpha demonstrated a major contribution of a 32-amino-acid 
      expansion in variable region 2 (v2) of the B30.2/SPRY domain to the breadth of 
      restriction. Data on lemur TRIM5alpha and the prediction of ancestral simian 
      sequences hint at an evolutionary scenario where antiretroviral specificity is 
      prominently defined by the lineage-specific expansion of the variable loops of 
      B30.2/SPRY.
FAU - Rahm, Nadia
AU  - Rahm N
AD  - Institute of Microbiology, University Hospital Center and University of Lausanne, 
      CHUV 1011 Lausanne, Switzerland.
FAU - Yap, Melvyn
AU  - Yap M
FAU - Snoeck, Joke
AU  - Snoeck J
FAU - Zoete, Vincent
AU  - Zoete V
FAU - Munoz, Miguel
AU  - Munoz M
FAU - Radespiel, Ute
AU  - Radespiel U
FAU - Zimmermann, Elke
AU  - Zimmermann E
FAU - Michielin, Olivier
AU  - Michielin O
FAU - Stoye, Jonathan P
AU  - Stoye JP
FAU - Ciuffi, Angela
AU  - Ciuffi A
FAU - Telenti, Amalio
AU  - Telenti A
LA  - eng
GR  - MC_U117512710/MRC_/Medical Research Council/United Kingdom
GR  - P41 RR001081/RR/NCRR NIH HHS/United States
GR  - U117512710/MRC_/Medical Research Council/United Kingdom
GR  - P41 RR-01081/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110223
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Carrier Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Carrier Proteins/chemistry/genetics/*metabolism
MH  - Cluster Analysis
MH  - Evolution, Molecular
MH  - Lemur/*immunology
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Protein Structure, Tertiary
MH  - Retroviridae/*immunology
MH  - Sequence Homology, Amino Acid
PMC - PMC3126249
EDAT- 2011/02/25 06:00
MHDA- 2011/06/15 06:00
PMCR- 2011/11/01
CRDT- 2011/02/25 06:00
PHST- 2011/02/25 06:00 [entrez]
PHST- 2011/02/25 06:00 [pubmed]
PHST- 2011/06/15 06:00 [medline]
PHST- 2011/11/01 00:00 [pmc-release]
AID - JVI.00075-11 [pii]
AID - 0075-11 [pii]
AID - 10.1128/JVI.00075-11 [doi]
PST - ppublish
SO  - J Virol. 2011 May;85(9):4173-83. doi: 10.1128/JVI.00075-11. Epub 2011 Feb 23.