PMID- 21346028
OWN - NLM
STAT- MEDLINE
DCOM- 20110712
LR  - 20200228
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Linking)
VI  - 92
IP  - Pt 6
DP  - 2011 Jun
TI  - Porcine reproductive and respiratory syndrome virus in Ontario, Canada 1999 to 
      2010: genetic diversity and restriction fragment length polymorphisms.
PG  - 1391-1397
LID - 10.1099/vir.0.030155-0 [doi]
AB  - Classification of Ontario porcine reproductive and respiratory syndrome virus 
      (PRRSV) field isolates (n = 505) from 1999 to 2010, based on a global type 2 
      PRRSV ORF5 phylogenetic framework, revealed genetic diversity comparable to PRRSV 
      in the USA, with sequences assigned to five of nine lineages (1, 2, 5, 8 and 9). 
      Importantly, the tree topology indicated a Canadian ancestry for the highly 
      virulent MN184-related strains that first emerged in 2001 in the USA. Mapping of 
      the RFLP patterns onto the phylogenetic tree revealed numerous examples of 
      different RFLP patterns located within the same phylogenetic cluster. Statistical 
      analysis showed occurrences where similar RFLP patterns masked diverse genetic 
      distances and instances of close genetic proximity with divergent RFLP patterns. 
      Collectively, extensive genetic diversity prevails in type 2 PRRSV in one region 
      of the North American swine industry, and it is not described adequately by RFLP 
      typing, which might have value in differentiating strains at the local farm 
      level.
FAU - Brar, Manreetpal Singh
AU  - Brar MS
AD  - School of Biological Sciences, The University of Hong Kong, Hong Kong SAR.
FAU - Shi, Mang
AU  - Shi M
AD  - School of Biological Sciences, The University of Hong Kong, Hong Kong SAR.
FAU - Ge, Li
AU  - Ge L
AD  - University of Guelph, Laboratory Services Division, Animal Health Laboratory, Box 
      3612, Guelph, ON N1H 6R8, Canada.
FAU - Carman, Susy
AU  - Carman S
AD  - University of Guelph, Laboratory Services Division, Animal Health Laboratory, Box 
      3612, Guelph, ON N1H 6R8, Canada.
FAU - Murtaugh, Michael P
AU  - Murtaugh MP
AD  - Department of Veterinary and Biomedical Sciences, St Paul, University of 
      Minnesota, MN, USA.
FAU - Leung, Frederick Chi-Ching
AU  - Leung FC
AD  - School of Biological Sciences, The University of Hong Kong, Hong Kong SAR.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110223
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
SB  - IM
MH  - Animals
MH  - *Genetic Variation
MH  - Molecular Sequence Data
MH  - Ontario
MH  - Phylogeny
MH  - *Polymorphism, Restriction Fragment Length
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/classification/*genetics/*isolation & purification
MH  - Swine
EDAT- 2011/02/25 06:00
MHDA- 2011/07/13 06:00
CRDT- 2011/02/25 06:00
PHST- 2011/02/25 06:00 [entrez]
PHST- 2011/02/25 06:00 [pubmed]
PHST- 2011/07/13 06:00 [medline]
AID - 10.1099/vir.0.030155-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2011 Jun;92(Pt 6):1391-1397. doi: 10.1099/vir.0.030155-0. Epub 2011 
      Feb 23.