PMID- 21349779
OWN - NLM
STAT- MEDLINE
DCOM- 20110630
LR  - 20110307
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 879
IP  - 9-10
DP  - 2011 Mar 15
TI  - Analysis of perfluorinated chemicals in umbilical cord blood by ultra-high 
      performance liquid chromatography/tandem mass spectrometry.
PG  - 641-6
LID - 10.1016/j.jchromb.2011.01.037 [doi]
AB  - Perfluorinated compounds (PFCs) can cross the placental barrier and enter fetal 
      circulation. This study aimed at developing a fast and sensitive ultra-high 
      performance liquid chromatography/tandem mass spectrometry method for the 
      determination of twelve perfluorinated compounds in cord blood. Samples were 
      processed with protein precipitation using formic acid and methanol, mixed with 
      stable isotope labeled standard, followed by sonication and centrifugation, and 
      were analyzed using a Waters ACQUITY UPLC coupled with a Waters Quattro Premier 
      XE triple-quadrupole mass spectrometer. The instrument was operated in selected 
      reaction monitoring (SRM) with negative electrospray ionization. Using BEH C(18) 
      column (2.1 mmx50 mm, 1.7 mum) with 10-mM N-methylmorpholine/methanol gradient 
      elution provided a fast chromatographic separation (5.5 min) and sharp peaks. 
      Intra- and inter-day calibration bias was less than 7% and intra- and inter-day 
      calibration of relative standard deviations were within 0.02-8.22% for all the 
      analytes and concentrations. The recoveries of PFCs spiked into bovine serum 
      ranged from 85 to 104% with relative standard deviations from 0.02 to 6.37%. The 
      limits of quantitation (LOQs), defined as a signal-to-noise ratio of ten, ranged 
      from 0.15 to 3.1 ng/mL for the twelve PFCs. Perfluorooctanoic acid (PFOA), 
      perfluorooctyl sulfonate (PFOS), perfluoroundecanoic acid (PFUA) and 
      perfluorononanoic acid (PFNA) were detected in up to 68% of umbilical cord plasma 
      (n=444) in Taiwan Birth Panel Study and the health effect of these chemicals on 
      children developmental deserves further investigation.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Lien, Guang-Wen
AU  - Lien GW
AD  - Institute of Occupational Medicine and Industrial Hygiene, Collage of Public 
      Health, National Taiwan University, Taipei 10055, Taiwan.
FAU - Wen, Ting-Wen
AU  - Wen TW
FAU - Hsieh, Wu-Shiun
AU  - Hsieh WS
FAU - Wu, Kuen-Yuh
AU  - Wu KY
FAU - Chen, Chia-Yang
AU  - Chen CY
FAU - Chen, Pau-Chung
AU  - Chen PC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110223
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Blood Proteins)
RN  - 0 (Fluorocarbons)
SB  - IM
MH  - Animals
MH  - Blood Proteins/isolation & purification
MH  - Cattle
MH  - Chemical Precipitation
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Fetal Blood/*chemistry
MH  - Fluorocarbons/*blood
MH  - Humans
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2011/02/26 06:00
MHDA- 2011/07/01 06:00
CRDT- 2011/02/26 06:00
PHST- 2010/09/09 00:00 [received]
PHST- 2010/12/27 00:00 [revised]
PHST- 2011/01/30 00:00 [accepted]
PHST- 2011/02/26 06:00 [entrez]
PHST- 2011/02/26 06:00 [pubmed]
PHST- 2011/07/01 06:00 [medline]
AID - S1570-0232(11)00071-7 [pii]
AID - 10.1016/j.jchromb.2011.01.037 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Mar 15;879(9-10):641-6. doi: 
      10.1016/j.jchromb.2011.01.037. Epub 2011 Feb 23.