PMID- 21351537
OWN - NLM
STAT- MEDLINE
DCOM- 20111115
LR  - 20181201
IS  - 1009-3591 (Print)
IS  - 1009-3591 (Linking)
VI  - 17
IP  - 1
DP  - 2011 Jan
TI  - [Effects of jiawei huzhang san decoction on the expressions of inflammatory 
      factors MCP-1 and PDGF-BB in rat models of experimental autoimmune prostatitis].
PG  - 83-8
AB  - OBJECTIVE: To study the effects of Jiawei Huzhang San (JWHZS) decoction on the 
      expressions of the inflammatory factors monocyte chemoattractant protein-1 
      (MCP-1) and platelet-derived growth factor-BB (PDGF-BB) on experimental 
      autoimmune prostatitis in rats. METHODS: Twelve male Wistar rats were taken as 
      normal controls, and models of experimental autoimmune prostatitis were 
      established in another 60 by injection of SC purified prostate protein with FCA, 
      and then divided into five groups to be treated with normal saline, indomethacin, 
      high-dose JWHZS (0.445 g/kg), medium-dose JWHZS (0.223 g/kg) and low-dose JWHZS 
      (0.089 g/kg), respectively. All the rats were sacrificed at 30 days after the 
      treatment for detection of the mRNA and protein expressions of inflammatory 
      factors by immunohistochemistry and fluorescent quantitative RT-PCR. RESULTS: In 
      the high-, medium- and low-dose JWHZS groups, the mRNA expressions of MCP-1 (0.31 
      +/- 0.14, 0.49 +/- 0.21 and 0.62 +/- 0.28) and PDGF-BB (0.50 +/- 0.22, 0.54 +/- 
      0.17 and 0.71 +/- 0.29), and the protein expressions of MCP-1 (677 +/- 208, 725 
      +/- 311 and 1302 +/- 884) and PDGF-BB (1265 +/- 698, 1347 +/- 827 and 1655 +/- 
      812) were significantly lower than in the model control group (MCP-1 mRNA: 1.12 
      +/- 0.43; MCP-1 protein: 2201 +/- 934; PDGF-BB mRNA: 1.14 +/- 0.51; PDGF-BB 
      protein: 2754 +/- 852) (P < 0.05). And JWHZS exhibited a significantly better 
      activity at high and medium doses than at a low dose (P < 0.05). In the 
      indomethacin control group, both the mRNA and protein expressions of MCP-1 (0.71 
      +/- 0.34 and 1824 +/- 1157) and PDGF-BB (1.08 +/- 0.37 and 2493 +/- 924) were 
      markedly higher than in the JWHZS groups (P < 0.01). CONCLUSION: Down-regulation 
      of the inflammatory factors MCP-1 and PDGF-BB may be the important molecular 
      mechanism of JWHZS acting on experimental autoimmune prostatitis.
FAU - Zhou, Qing
AU  - Zhou Q
AD  - Department of Surgery, The First Affiliated Hospital of Hunan University of 
      Chinese Medicine, Changsha, Hunan 410007, China. supergoon@163.com
FAU - Tian, Xue-fei
AU  - Tian XF
FAU - Gong, Xiu-ying
AU  - Gong XY
FAU - Feng, Ya-lan
AU  - Feng YL
FAU - Hu, Hua
AU  - Hu H
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Nan Ke Xue
JT  - Zhonghua nan ke xue = National journal of andrology
JID - 101093592
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (Proto-Oncogene Proteins c-sis)
RN  - 0 (RNA, Messenger)
RN  - 1B56C968OA (Becaplermin)
SB  - IM
MH  - Animals
MH  - Autoimmune Diseases/*drug therapy/metabolism
MH  - Becaplermin
MH  - Chemokine CCL2/*metabolism
MH  - Disease Models, Animal
MH  - Drugs, Chinese Herbal/*therapeutic use
MH  - Inflammation
MH  - Male
MH  - *Phytotherapy
MH  - Platelet-Derived Growth Factor/*metabolism
MH  - Prostatitis/*drug therapy/metabolism
MH  - Proto-Oncogene Proteins c-sis
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Wistar
EDAT- 2011/03/01 06:00
MHDA- 2011/11/16 06:00
CRDT- 2011/03/01 06:00
PHST- 2011/03/01 06:00 [entrez]
PHST- 2011/03/01 06:00 [pubmed]
PHST- 2011/11/16 06:00 [medline]
PST - ppublish
SO  - Zhonghua Nan Ke Xue. 2011 Jan;17(1):83-8.