PMID- 21354629
OWN - NLM
STAT- MEDLINE
DCOM- 20110729
LR  - 20110408
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 141
IP  - 1-2
DP  - 2011 May 15
TI  - Stability of expression of reference genes in porcine peripheral blood 
      mononuclear and dendritic cells.
PG  - 11-5
LID - 10.1016/j.vetimm.2011.01.005 [doi]
AB  - Real-time quantitative PCR (RT-qPCR) is a critical tool used to evaluate changes 
      in gene expression. The precision of this tool is reliant upon the selection of 
      reference genes whose expression remains unaltered in culture conditions and 
      following stimulation. Stably expressed reference genes are used to normalize 
      data so observed changes in expression are not due to artifacts but rather 
      reflect physiological changes. In this study, we examined the expression 
      stability of the porcine genes glyceraldehyde 3-phosphate dehydrogenase (GAPDH), 
      succinate dehydrogenase complex subunit A (SDHA), eukaryotic elongation factor 1 
      gamma-like protein (eEF1), ribosomal protein L19 (RPL19), beta-actin (ACTB) and 
      ATP synthase mitochondrial F0 complex (ATP5G1) in peripheral blood mononuclear 
      cells (PBMCs), monocytes, monocyte-derived dendritic cells (MoDCs), blood 
      isolated dendritic cells (BDCs) and T cells with or without stimulation with 
      lipolysaccharide (LPS). An M value was used as a measure of gene stability as 
      determined using geNORM software. Recommendations for the use of reference genes 
      include using GAPDH and B-actin in PBMCs: RPL19 and SDHA in T cells; RPL19 and 
      B-actin in monocytes; RPL-19 and SDHA in BDCs: and RPL-19 and ATP5GA in MoDCs.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Facci, Marina R
AU  - Facci MR
AD  - Vaccine & Infectious Disease Organization, University of Saskatchewan, Saskatoon 
      S7N5E3, Canada.
FAU - Auray, Gael
AU  - Auray G
FAU - Meurens, Francois
AU  - Meurens F
FAU - Buchanan, Rachelle
AU  - Buchanan R
FAU - van Kessel, Jill
AU  - van Kessel J
FAU - Gerdts, Volker
AU  - Gerdts V
LA  - eng
PT  - Journal Article
DEP - 20110113
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Actins)
RN  - 0 (Eukaryotic Initiation Factor-1)
RN  - 0 (RNA, Messenger)
RN  - 0 (Ribosomal Proteins)
RN  - EC 1.2.1.9 (Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+))
RN  - EC 1.3.99.1 (Succinate Dehydrogenase)
RN  - EC 3.6.3.- (Mitochondrial Proton-Translocating ATPases)
SB  - IM
MH  - Actins/biosynthesis/genetics
MH  - Animals
MH  - Dendritic Cells/*immunology
MH  - Eukaryotic Initiation Factor-1/biosynthesis/genetics
MH  - Gene Expression Regulation/*immunology
MH  - Genes/genetics
MH  - Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)/biosynthesis/genetics
MH  - Leukocytes, Mononuclear/*immunology
MH  - Mitochondrial Proton-Translocating ATPases/biosynthesis/genetics
MH  - RNA, Messenger/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Ribosomal Proteins/biosynthesis/genetics
MH  - Succinate Dehydrogenase/biosynthesis/genetics
MH  - Swine/genetics/immunology
EDAT- 2011/03/01 06:00
MHDA- 2011/07/30 06:00
CRDT- 2011/03/01 06:00
PHST- 2010/06/21 00:00 [received]
PHST- 2011/01/03 00:00 [revised]
PHST- 2011/01/05 00:00 [accepted]
PHST- 2011/03/01 06:00 [entrez]
PHST- 2011/03/01 06:00 [pubmed]
PHST- 2011/07/30 06:00 [medline]
AID - S0165-2427(11)00012-2 [pii]
AID - 10.1016/j.vetimm.2011.01.005 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2011 May 15;141(1-2):11-5. doi: 
      10.1016/j.vetimm.2011.01.005. Epub 2011 Jan 13.