PMID- 21355095
OWN - NLM
STAT- MEDLINE
DCOM- 20110713
LR  - 20220408
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 117
IP  - 16
DP  - 2011 Apr 21
TI  - miR-221 and miR-155 regulate human dendritic cell development, apoptosis, and 
      IL-12 production through targeting of p27kip1, KPC1, and SOCS-1.
PG  - 4293-303
LID - 10.1182/blood-2010-12-322503 [doi]
AB  - Dendritic cells (DCs) are potent antigen-presenting cells derived from 
      hematopoietic progenitor cells and circulating monocytes. To investigate the role 
      of microRNAs (miRNAs) during DC differentiation, maturation, and function, we 
      profiled miRNA expression in human monocytes, immature DCs (imDCs), and mature 
      DCs (mDCs). Stage-specific, differential expression of 27 miRNAs was found during 
      monocyte differentiation into imDCs and mDCs. Among them, decreased miR-221 and 
      increased miR-155 expression correlated with p27(kip1) accumulation in DCs. 
      Silencing of miR-221 or overexpressing of miR-155 in DCs resulted in p27(kip1) 
      protein increase and DC apoptosis. Moreover, mDCs from miR-155(-/-) mice were 
      less apoptotic than those from wild-type mice. Silencing of miR-155 expression 
      had little effect on DC maturation but reduced IL-12p70 production, whereas 
      miR-155 overexpression in mDCs enhanced IL-12p70 production. Kip1 
      ubiquitination-promoting complex 1, suppressor of cytokine signaling 1, and CD115 
      (M-CSFR) were functional targets of miR-155. Furthermore, we provide evidence 
      that miR-155 indirectly regulated p27(kip1) protein level by targeting Kip1 
      ubiquitination-promoting complex 1. Thus, our study uncovered miRNA signatures 
      during monocyte differentiation into DCs and the new regulatory role of miR-221 
      and miR-155 in DC apoptosis and IL-12p70 production.
FAU - Lu, Changming
AU  - Lu C
AD  - Pediatric Blood and Marrow Transplantation, University of Minnesota Medical 
      School, Minneapolis, MN 55455, USA.
FAU - Huang, Xin
AU  - Huang X
FAU - Zhang, Xiaoxiao
AU  - Zhang X
FAU - Roensch, Kristin
AU  - Roensch K
FAU - Cao, Qing
AU  - Cao Q
FAU - Nakayama, Keiichi I
AU  - Nakayama KI
FAU - Blazar, Bruce R
AU  - Blazar BR
FAU - Zeng, Yan
AU  - Zeng Y
FAU - Zhou, Xianzheng
AU  - Zhou X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110225
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (CDKN1B protein, human)
RN  - 0 (MIRN155 microRNA, human)
RN  - 0 (MIRN221 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (SOCS1 protein, human)
RN  - 0 (Suppressor of Cytokine Signaling 1 Protein)
RN  - 0 (Suppressor of Cytokine Signaling Proteins)
RN  - 147604-94-2 (Cyclin-Dependent Kinase Inhibitor p27)
RN  - 187348-17-0 (Interleukin-12)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 2.3.2.27 (RNF123 protein, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 2.7.10.1 (Receptor, Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Cells, Cultured
MH  - Cyclin-Dependent Kinase Inhibitor p27/*immunology
MH  - Dendritic Cells/*cytology/immunology/metabolism
MH  - Gene Expression
MH  - Humans
MH  - Interferon-gamma/immunology
MH  - Interleukin-12/*immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/genetics/*immunology
MH  - Receptor, Macrophage Colony-Stimulating Factor/immunology
MH  - Suppressor of Cytokine Signaling 1 Protein
MH  - Suppressor of Cytokine Signaling Proteins/*immunology
MH  - Ubiquitin-Protein Ligases/*immunology
PMC - PMC3087479
EDAT- 2011/03/01 06:00
MHDA- 2011/07/14 06:00
PMCR- 2012/04/21
CRDT- 2011/03/01 06:00
PHST- 2011/03/01 06:00 [entrez]
PHST- 2011/03/01 06:00 [pubmed]
PHST- 2011/07/14 06:00 [medline]
PHST- 2012/04/21 00:00 [pmc-release]
AID - S0006-4971(20)45241-3 [pii]
AID - 2010/322503 [pii]
AID - 10.1182/blood-2010-12-322503 [doi]
PST - ppublish
SO  - Blood. 2011 Apr 21;117(16):4293-303. doi: 10.1182/blood-2010-12-322503. Epub 2011 
      Feb 25.