PMID- 21355294
OWN - NLM
STAT- MEDLINE
DCOM- 20120117
LR  - 20221207
IS  - 1672-173X (Print)
IS  - 1672-173X (Linking)
VI  - 42
IP  - 1
DP  - 2011 Jan
TI  - [The -108 C/T polymorphism in paraoxonase 1 gene in Chinese patients with 
      polycystic ovary syndrome].
PG  - 24-8
AB  - OBJECTIVE: To investigate the relationship between the -108 C/T polymorphism in 
      the promoter of paraoxonase 1 (PON1) gene and polycystic ovarian syndrome (PCOS) 
      in Chinese women. METHODS: A total of six hundred and sixty one subjects (346 
      patients with PCOS and 315 healthy control women) in Chengdu area were included 
      in this study. The -108 C/T polymorphism in PON1 gene was studied using 
      polymerase chain reaction and restriction fragment length polymorphism analysis 
      (PCR-RFLP). Total testosterone (TT), luteinizing hormone (LH), follicle 
      stimulating hormone (FSH) and plasma insulin (INS) were determined by 
      chemiluminescent method. Plasma glucose (GLU), total cholesterol (TC), 
      triglycerides (TG), high density lipoprotein cholesterol (HDL-C), and low density 
      lipoprotein cholesterol (LDL-C) were measured by enzymatic assay. Serum 
      malondialdehyde (MDA) levels were measured by spectrophometry at 532 nm using 
      micro-MDA detection kits. RESULTS: The frequencies of CC, CT and TT genotypes at 
      PON1 -108 C/T site in PCOS and control groups were 0.283 and 0.324, 0.517 and 
      0.492, 0.199 and 0.184, respectively, and the frequencies of C and T alleles in 
      both groups were 0.542 and 0.570, 0.458 and 0.430, respectively. There were no 
      significant differences in the frequencies of the genotypes and alleles between 
      PCOS and control groups (P > 0.05). In the control group, the subjects with 
      genotype CT had higher F-G scores and plasma glucose levels at 2 h during OGTT 
      (75 g glucose) when compared with those of genotype CC (P < 0.05). Higher FSH 
      level and MDA concentration also observed in those of genotype TT by compared 
      with those of CC genotype (P < 0.05). The differences in FSH, GLU120 and MDA 
      levels remained significant after adjustment for age and BMI (P = 0.006, 0.033 
      and 0.047, respectively). In the PCOS group, the patients with genotype CT had 
      higher atherogenic index (AI) when compared with those of genotype CC (P = 
      0.038), but this difference was not significant after adjustment for age and BMI 
      (P = 0.053). CONCLUSION: This study indicated that the -108 C/T polymorphism in 
      the promoter of PON1 gene was associated with hormonal levels, glucose metabolism 
      and oxidative stress in the control women, but not associated with PCOS in 
      Chinese women of Chengdu area.
FAU - Zhang, Feng
AU  - Zhang F
AD  - Department of Obstertrics and Gynecology, West China Second Hospital, Sichuan 
      University, Chengdu 610041, China.
FAU - Liu, Hong-Wei
AU  - Liu HW
FAU - Fan, Ping
AU  - Fan P
FAU - Bai, Huai
AU  - Bai H
FAU - Song, Qi
AU  - Song Q
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sichuan Da Xue Xue Bao Yi Xue Ban
JT  - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical 
      science edition
JID - 101162609
RN  - EC 3.1.8.1 (Aryldialkylphosphatase)
RN  - EC 3.1.8.1 (PON1 protein, human)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aryldialkylphosphatase/*genetics
MH  - Asian People/genetics
MH  - Case-Control Studies
MH  - Female
MH  - Genotype
MH  - Humans
MH  - Polycystic Ovary Syndrome/*genetics
MH  - *Polymorphism, Genetic
MH  - Promoter Regions, Genetic/genetics
MH  - Young Adult
EDAT- 2011/03/02 06:00
MHDA- 2012/01/18 06:00
CRDT- 2011/03/02 06:00
PHST- 2011/03/02 06:00 [entrez]
PHST- 2011/03/02 06:00 [pubmed]
PHST- 2012/01/18 06:00 [medline]
PST - ppublish
SO  - Sichuan Da Xue Xue Bao Yi Xue Ban. 2011 Jan;42(1):24-8.