PMID- 21355310
OWN - NLM
STAT- MEDLINE
DCOM- 20120117
LR  - 20131121
IS  - 1672-173X (Print)
IS  - 1672-173X (Linking)
VI  - 42
IP  - 1
DP  - 2011 Jan
TI  - [Influence of high glucose and mannose binding lectin complement pathway 
      activation to IL-6 and TNF-alpha's expression by human renal glomerular 
      endothelial cells].
PG  - 90-4
AB  - OBJECTIVE: To investigate the effect of high glucose and mannose binding lectin 
      (MBL) complement pathway activation's effect on expression of Interleukin-6 
      (IL-6) and Tumor necrosis factor-alpha (TNF-alpha) from human renal glomerular 
      endothelial cells (HRGEC), to explore unknown pathogenesy of diabetic 
      nephropathy. METHODS: Normal HRGEC was divided randomly into normal glucose 
      group(5 mmol/L D-glucose), manicol group (5 mmol/L D-glucose+25 mmol/L manicol) 
      and high glucose group (30 mmol/L D-glucose). Real-time PCR was used to detect 
      IL-6 and TNF-alpha mRNA expression in each group, Euzymelinked Immunosorbent 
      Assay (ELISA) was performed to examine the protein expression of IL-6 and 
      TNF-alpha in supernatant after 24 hours' culture. HRGEC was then randomly divided 
      into two groups: single high glucose group and high glucose + MBL group. After 24 
      hours' culture with 30 mmol/L D-glucose, 30% MBL deficiency human serum was added 
      into two groups, 1 microg/mL MBL was only added into high glucose + MBL group, 
      continued the culturation for another 4 hours. Flow cytometry and 
      immunofluorescence technique were applied to evaluate MBL, C3 and membrane 
      attacks complex (MAC) deposition on cell surface respectively. Real-time PCR and 
      ELISA were performed to examine mRNA and protein expression of both IL-6 and 
      TNF-alpha in each group. RESULTS: Compared with normal glucose group and manicol 
      group, the mRNA and protein expression of IL-6 and TNF-alpha in high glucose 
      group were increased (P < 0.05). Flow cytometry confirmed obvious MBL and C3 
      co-deposition and Immunofluorescence confirmed obvious MAC deposition on cell 
      surface in high glucose+ MBL group. Compared with single high glucose group, the 
      mRNA and protein expression of IL-6 and TNF-alpha in high glucose+ MBL group were 
      significantly higher (P < 0.05). CONCLUSION: High glucose can bring inflammatory 
      factors' overexpression from cultured HRGEC; high glucose together with MBL can 
      bring MBL complement pathway activation and inflammatory factors' overexpression, 
      this indicates that the activation of MBL complement pathway may be a potential 
      unknown pathogenesy of diabetic nephropathy and its proinflammatory status.
FAU - Wu, Xiao-hui
AU  - Wu XH
AD  - Department of Nephrology, West China Hospital, Sichuan University, Chengdu 
      610041, China.
FAU - Huang, Song-min
AU  - Huang SM
FAU - Fan, Wen-xing
AU  - Fan WX
FAU - Tang, Wan-xin
AU  - Tang WX
FAU - Qiu, Hong-yu
AU  - Qiu HY
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sichuan Da Xue Xue Bao Yi Xue Ban
JT  - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical 
      science edition
JID - 101162609
RN  - 0 (Interleukin-6)
RN  - 0 (Mannose-Binding Lectin)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Cells, Cultured
MH  - Complement Pathway, Mannose-Binding Lectin/*physiology
MH  - Diabetic Nephropathies/etiology
MH  - Endothelial Cells/cytology/metabolism
MH  - Glucose/*pharmacology
MH  - Humans
MH  - Interleukin-6/genetics/*metabolism
MH  - Kidney Glomerulus/*cytology/metabolism
MH  - Mannose-Binding Lectin/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Tumor Necrosis Factor-alpha/genetics/*metabolism
EDAT- 2011/03/02 06:00
MHDA- 2012/01/18 06:00
CRDT- 2011/03/02 06:00
PHST- 2011/03/02 06:00 [entrez]
PHST- 2011/03/02 06:00 [pubmed]
PHST- 2012/01/18 06:00 [medline]
PST - ppublish
SO  - Sichuan Da Xue Xue Bao Yi Xue Ban. 2011 Jan;42(1):90-4.