PMID- 21355864
OWN - NLM
STAT- MEDLINE
DCOM- 20110527
LR  - 20211020
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Print)
IS  - 0019-2805 (Linking)
VI  - 133
IP  - 1
DP  - 2011 May
TI  - Allogeneic apoptotic thymocyte-stimulated dendritic cells expand functional 
      regulatory T cells.
PG  - 123-32
LID - 10.1111/j.1365-2567.2011.03420.x [doi]
AB  - Dendritic cells (DCs) play an important role in the clearance of apoptotic cells. 
      The removal of apoptotic cells leads to peripheral tolerance, although their role 
      is still not clear. We show that the uptake of apoptotic thymocytes by DCs 
      converts these cells into tolerogenic DCs resistant to maturation by 
      lipopolysaccharide, modulating the production of interleukin-12 and up-regulating 
      the expression of transforming growth factor-beta(1) latency associated peptide. We 
      also observed that DCs pulsed with apoptotic cells in the allogeneic context were 
      more efficient in the expansion of regulatory T cells (Tregs), and that this 
      expansion requires contact between DCs and the T cell. The Tregs sorted from in 
      vitro culture suppressed the proliferation of splenocytes in vitro in a specific 
      and non-specific manner. In the in vivo model, the transfer of CD4(+) CD25(-) 
      cells to Nude mice induced autoimmunity, with cell infiltrate found in the 
      stomach, colon, liver and kidneys. The co-transfer of CD4(+) CD25(-) and CD4(+) 
      CD25(+) prevented the presence of cell infiltrates in several organs and 
      increased the total cell count in lymph nodes. Our data indicate that apoptotic 
      cells have an important role in peripheral tolerance via induction of tolerogenic 
      DCs and CD4(+) CD25(+) Foxp3(+) cells that present regulatory functions.
CI  - (c) 2011 The Authors. Immunology (c) 2011 Blackwell Publishing Ltd.
FAU - da Costa, Thais Boccia
AU  - da Costa TB
AD  - Department of Immunology, Institute of Biomedical Sciences, University of Sao 
      Paulo, Sao Paulo, Brazil. thais.boccia@gmail.com
FAU - Sardinha, Luiz R
AU  - Sardinha LR
FAU - Larocca, Rafael
AU  - Larocca R
FAU - Peron, Jean P S
AU  - Peron JP
FAU - Rizzo, Luiz V
AU  - Rizzo LV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110301
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
SB  - IM
MH  - Animals
MH  - Apoptosis/*immunology
MH  - Cell Separation
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Flow Cytometry
MH  - Immune Tolerance/*immunology
MH  - Lymphocyte Activation/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - T-Lymphocytes, Regulatory/*immunology
PMC - PMC3088974
EDAT- 2011/03/02 06:00
MHDA- 2011/05/28 06:00
PMCR- 2012/05/01
CRDT- 2011/03/02 06:00
PHST- 2011/03/02 06:00 [entrez]
PHST- 2011/03/02 06:00 [pubmed]
PHST- 2011/05/28 06:00 [medline]
PHST- 2012/05/01 00:00 [pmc-release]
AID - 10.1111/j.1365-2567.2011.03420.x [doi]
PST - ppublish
SO  - Immunology. 2011 May;133(1):123-32. doi: 10.1111/j.1365-2567.2011.03420.x. Epub 
      2011 Mar 1.