PMID- 21356235
OWN - NLM
STAT- MEDLINE
DCOM- 20110901
LR  - 20131121
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 48
IP  - 5
DP  - 2011 Jun 1
TI  - Chitooligosaccharide-mediated neuroprotection is associated with modulation of 
      Hsps expression and reduction of MAPK phosphorylation.
PG  - 726-35
LID - 10.1016/j.ijbiomac.2011.02.011 [doi]
AB  - There is mounting evidence implicating the role of oxidative stress induced by 
      reactive oxygen species (ROS) in neurodegenerative disease, including Alzheimer's 
      disease. In this study we aimed to investigate the possible protective effect of 
      chitooligosaccharide (COS), an antioxidant oligosaccharide, on hydrogen peroxide 
      induced apoptosis in NGF-differentitated rat pheochromocytoma (PC12) cells. COS 
      treatment reversed the decrease of cell viability induced by H(2)O(2) and this 
      was associated with diminished intracellular ROS and decreased level of cytosolic 
      Ca(2+). Additionally, COS contributed to up-regulation of Bcl-2, down regulation 
      of Bax protein and reduction of cleaved Caspase-3 protein. COS treatment 
      stabilized Nrf2 in nucleus and increased the Hsp70 level within cell while 
      down-regulated Hsp90 expression. Moreover, COS could inhibit the phosphorylation 
      of different mitogen activated protein kinases (MAPKs), whose aberrant 
      phosphorylation has been implicated in AD. Our findings suggest that heat shock 
      response and MAPK cascades are both involved in cell survival, and by 
      concomitantly regulating both pathways, COS can be a promising agent in treating 
      neurodegenerative diseases.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Joodi, Golsa
AU  - Joodi G
AD  - Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, 
      Tehran, Iran.
FAU - Ansari, Niloufar
AU  - Ansari N
FAU - Khodagholi, Fariba
AU  - Khodagholi F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110226
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Antioxidants)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 9012-76-4 (Chitosan)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.4.22.- (Caspase 3)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Apoptosis/drug effects
MH  - Calcium/metabolism
MH  - Caspase 3/metabolism
MH  - Cell Differentiation/drug effects
MH  - Cell Line, Tumor
MH  - Chitosan/*analogs & derivatives/*pharmacology
MH  - Enzyme Activation/drug effects
MH  - *Gene Expression Regulation/drug effects
MH  - Heat-Shock Proteins/*metabolism
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - NF-E2-Related Factor 2/metabolism
MH  - Neuroprotective Agents/*pharmacology
MH  - Oxidative Stress/physiology
MH  - Phosphorylation/drug effects
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - Rats
MH  - Reactive Oxygen Species/metabolism
MH  - Superoxide Dismutase/metabolism
MH  - bcl-2-Associated X Protein/metabolism
EDAT- 2011/03/02 06:00
MHDA- 2011/09/02 06:00
CRDT- 2011/03/02 06:00
PHST- 2010/12/18 00:00 [received]
PHST- 2011/02/14 00:00 [revised]
PHST- 2011/02/21 00:00 [accepted]
PHST- 2011/03/02 06:00 [entrez]
PHST- 2011/03/02 06:00 [pubmed]
PHST- 2011/09/02 06:00 [medline]
AID - S0141-8130(11)00079-1 [pii]
AID - 10.1016/j.ijbiomac.2011.02.011 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2011 Jun 1;48(5):726-35. doi: 
      10.1016/j.ijbiomac.2011.02.011. Epub 2011 Feb 26.