PMID- 21356908
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20200930
IS  - 1559-6095 (Linking)
VI  - 2008
DP  - 2008 Sep 1
TI  - Human lymphocyte culture and chromosome analysis.
PG  - pdb.prot5035
LID - 2008/9/pdb.prot5035 [pii]
LID - 10.1101/pdb.prot5035 [doi]
AB  - INTRODUCTIONPhytohaemagglutinin (PHA), a lectin derived from the red kidney bean, 
      is a powerful mitogen for human T-cells. When PHA is added in vitro to whole 
      blood, mitotic cells can be found after 48 h, with a peak mitotic index at ~64-72 
      h. The convenience of peripheral blood as a source of human cells, the abundance 
      of mitotic cells, and the simplicity of the cell culture technique make this the 
      most convenient approach to study human chromosomes for both clinical and 
      research purposes. This method of chromosome preparation provides metaphase cells 
      that can be stained by a variety of methods or used for fluorescence in situ 
      hybridization (FISH). The most common chromosome staining techniques involve 
      exposing fixed preparations to a protease (e.g., trypsin), followed by an 
      appropriate semipermanent stain. The characteristic banding patterns obtained 
      reflect both structural and functional differences in different parts of the 
      chromosomes. The staining procedure described here provides a Giemsa banding 
      pattern using trypsin with Wright stain (i.e., GTW banding). This procedure is 
      reliable and, with only minor modifications, suitable for preparing chromosomes 
      from a variety of human tissues.
FAU - Benn, Peter
AU  - Benn P
AD  - Human Genetics Laboratories, Department of Genetics and Developmental Biology, 
      and Department of Pathology and Laboratory Medicine, University of Connecticut 
      Health Center, Farmington, CT 06030-6140, USA.
FAU - Delach, Judith
AU  - Delach J
LA  - eng
PT  - Journal Article
DEP - 20080901
PL  - United States
TA  - CSH Protoc
JT  - CSH protocols
JID - 101280522
EDAT- 2008/01/01 00:00
MHDA- 2008/01/01 00:01
CRDT- 2011/03/02 06:00
PHST- 2011/03/02 06:00 [entrez]
PHST- 2008/01/01 00:00 [pubmed]
PHST- 2008/01/01 00:01 [medline]
AID - 2008/9/pdb.prot5035 [pii]
AID - 10.1101/pdb.prot5035 [doi]
PST - epublish
SO  - CSH Protoc. 2008 Sep 1;2008:pdb.prot5035. doi: 10.1101/pdb.prot5035.