PMID- 21357074
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20200929
IS  - 1559-6095 (Linking)
VI  - 2007
DP  - 2007 May 1
TI  - FISH on Histological Sections.
PG  - pdb.prot4729
LID - 2007/5/pdb.prot4729 [pii]
LID - 10.1101/pdb.prot4729 [doi]
AB  - IntroductionHere we describe fluorescence in situ hybridization (FISH) of DNA 
      probes to histological sections, which allows the visualization of specific DNA 
      targets (chromosome territories and their subregions) in the context of 
      functional tissue organization. Separate protocols are provided for hybridization 
      using paraffin-embedded tissue sections and for hybridization using vibratome or 
      frozen sections. Pretreatment with heat or protease is necessary to allow 
      unmasking of the target DNA and efficient penetration of reagents in the nuclei. 
      Because the goal of the technique is to obtain data on the native 3D structure of 
      the genome, close attention is paid to the preservation of nuclear morphology.
FAU - Solovei, Irina
AU  - Solovei I
AD  - Ludwig-Maximilians University Munich, Department of Biology II, AG Thomas Cremer 
      (Chair of Anthropology and Human Genetics), 82152 Martinsried-Planegg, Germany.
FAU - Grasser, Florian
AU  - Grasser F
FAU - Lanctot, Christian
AU  - Lanctot C
LA  - eng
PT  - Journal Article
DEP - 20070501
PL  - United States
TA  - CSH Protoc
JT  - CSH protocols
JID - 101280522
EDAT- 2007/01/01 00:00
MHDA- 2007/01/01 00:01
CRDT- 2011/03/02 06:00
PHST- 2011/03/02 06:00 [entrez]
PHST- 2007/01/01 00:00 [pubmed]
PHST- 2007/01/01 00:01 [medline]
AID - 2007/5/pdb.prot4729 [pii]
AID - 10.1101/pdb.prot4729 [doi]
PST - epublish
SO  - CSH Protoc. 2007 May 1;2007:pdb.prot4729. doi: 10.1101/pdb.prot4729.