PMID- 21357075
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20200930
IS  - 1559-6095 (Linking)
VI  - 2007
DP  - 2007 May 1
TI  - Preparation of Complex DNA Probe Sets for 3D FISH with up to Six Different 
      Fluorochromes.
PG  - pdb.prot4730
LID - 2007/5/pdb.prot4730 [pii]
LID - 10.1101/pdb.prot4730 [doi]
AB  - INTRODUCTIONDNA probes for fluorescence in situ hybridization (FISH) can be 
      generated and labeled by various methods. This protocol describes the conjugation 
      of dUTPs with haptens or fluorochromes, as well as the generation and labeling of 
      DNA probes using those modified dUTPs. Sources of probe DNA include genomic DNA, 
      DNA from flow-sorted chromosomes, bacterial artificial chromosomes (BACs), and 
      cosmids. DNA amplification and labeling procedures involving degenerate 
      oligonucleotide-primed PCR (DOP-PCR) and multiple displacement amplification 
      (MDA) are provided. Advice is given for setting up complex probe pools, such as 
      those containing large pools of BAC probes. Also included is a method for probe 
      precipitation and preparation of a hybridization mix ready to be used for 3D 
      fluorescence in situ hybridization (FISH) experiments.
FAU - Muller, Stefan
AU  - Muller S
AD  - Ludwig-Maximilians University Munich, Department Biology II, AG Thomas Cremer 
      (Chair of Anthropology and Human Genetics), 82152 Martinsried-Planegg, Germany.
FAU - Neusser, Michaela
AU  - Neusser M
FAU - Kohler, Daniela
AU  - Kohler D
FAU - Cremer, Marion
AU  - Cremer M
LA  - eng
PT  - Journal Article
DEP - 20070501
PL  - United States
TA  - CSH Protoc
JT  - CSH protocols
JID - 101280522
EDAT- 2007/01/01 00:00
MHDA- 2007/01/01 00:01
CRDT- 2011/03/02 06:00
PHST- 2011/03/02 06:00 [entrez]
PHST- 2007/01/01 00:00 [pubmed]
PHST- 2007/01/01 00:01 [medline]
AID - 2007/5/pdb.prot4730 [pii]
AID - 10.1101/pdb.prot4730 [doi]
PST - epublish
SO  - CSH Protoc. 2007 May 1;2007:pdb.prot4730. doi: 10.1101/pdb.prot4730.