PMID- 21357536
OWN - NLM
STAT- MEDLINE
DCOM- 20110616
LR  - 20211020
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 186
IP  - 7
DP  - 2011 Apr 1
TI  - Defining the herpes simplex virus-specific CD8+ T cell repertoire in C57BL/6 
      mice.
PG  - 3927-33
LID - 10.4049/jimmunol.1003735 [doi]
AB  - HSV type 1 (HSV-1) expresses its genes sequentially as immediate early (alpha), early 
      (beta), leaky late (gamma1), and true late (gamma2), where viral DNA synthesis is an 
      absolute prerequisite only for gamma2 gene expression. The gamma1 protein glycoprotein B 
      (gB) contains a strongly immunodominant CD8(+) T cell epitope (gB(498-505)) that 
      is recognized by 50% of both the CD8(+) effector T cells in acutely infected 
      trigeminal ganglia (TG) and the CD8(+) memory T cells in latently infected TG. Of 
      376 predicted HSV-1 CD8(+) T cell epitopes in C57BL/6 mice, 19 (gB(498-505) and 
      18 subdominant epitopes) stimulated CD8(+) T cells in the spleens and TG of HSV-1 
      acutely infected mice. These 19 epitopes identified virtually all CD8(+) T cells 
      in the infected TG that represent all or the vast majority of the HSV-specific 
      CD8(+) TCR repertoire. Only 11 of  approximately 84 HSV-1 proteins are recognized by CD8(+) T 
      cells, and most ( approximately 80%) are expressed before viral DNA synthesis. Neither the 
      immunodominance of gB(498-505) nor the dominance hierarchy of the subdominant 
      epitopes is due solely to MHC or TCR affinity. We conclude that the vast majority 
      of CD8(+) T cells in HSV-1 acutely infected TG are HSV specific, that HSV-1 beta and 
      gamma1 proteins that are expressed before viral DNA synthesis are favored targets of 
      CD8(+) T cells, and that dominance within the TCR repertoire is likely due to the 
      frequency or expansion and survival characteristics of CD8(+) T cell precursors.
FAU - St Leger, Anthony J
AU  - St Leger AJ
AD  - Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
FAU - Peters, Bjoern
AU  - Peters B
FAU - Sidney, John
AU  - Sidney J
FAU - Sette, Alessandro
AU  - Sette A
FAU - Hendricks, Robert L
AU  - Hendricks RL
LA  - eng
GR  - R01 EY005945-24/EY/NEI NIH HHS/United States
GR  - P30 EY008098-23/EY/NEI NIH HHS/United States
GR  - R01 EY005945/EY/NEI NIH HHS/United States
GR  - R01 EY005945-25/EY/NEI NIH HHS/United States
GR  - R01-EY005945/EY/NEI NIH HHS/United States
GR  - P30 EY008098-24/EY/NEI NIH HHS/United States
GR  - P30 EY008098-21/EY/NEI NIH HHS/United States
GR  - T32-EY017271/EY/NEI NIH HHS/United States
GR  - T32 EY017271/EY/NEI NIH HHS/United States
GR  - HHSN272200700048C/AI/NIAID NIH HHS/United States
GR  - R01 EY005945-27/EY/NEI NIH HHS/United States
GR  - P30-EY08098/EY/NEI NIH HHS/United States
GR  - R01 EY005945-26/EY/NEI NIH HHS/United States
GR  - P30 EY008098-22/EY/NEI NIH HHS/United States
GR  - P30 EY008098/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110225
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (Immunodominant Epitopes)
RN  - 0 (Receptors, Antigen, T-Cell)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - CD8-Positive T-Lymphocytes/*immunology/metabolism/virology
MH  - Cell Line
MH  - Cells, Cultured
MH  - Epitopes, T-Lymphocyte/*immunology/isolation & purification
MH  - Female
MH  - Herpes Simplex/immunology/pathology/virology
MH  - Herpesvirus 1, Human/growth & development/*immunology
MH  - Immunodominant Epitopes/biosynthesis/*immunology/isolation & purification
MH  - Keratitis, Herpetic/immunology/metabolism/virology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Receptors, Antigen, T-Cell/biosynthesis
MH  - Trigeminal Ganglion/immunology/metabolism/virology
MH  - Virus Latency/immunology
PMC - PMC3308013
MID - NIHMS353429
EDAT- 2011/03/02 06:00
MHDA- 2011/06/17 06:00
PMCR- 2012/03/20
CRDT- 2011/03/02 06:00
PHST- 2011/03/02 06:00 [entrez]
PHST- 2011/03/02 06:00 [pubmed]
PHST- 2011/06/17 06:00 [medline]
PHST- 2012/03/20 00:00 [pmc-release]
AID - jimmunol.1003735 [pii]
AID - 10.4049/jimmunol.1003735 [doi]
PST - ppublish
SO  - J Immunol. 2011 Apr 1;186(7):3927-33. doi: 10.4049/jimmunol.1003735. Epub 2011 
      Feb 25.