PMID- 21360641
OWN - NLM
STAT- MEDLINE
DCOM- 20110624
LR  - 20161125
IS  - 1521-6551 (Electronic)
IS  - 1521-6543 (Linking)
VI  - 63
IP  - 2
DP  - 2011 Feb
TI  - Effect of hypoxia on the expression of iron regulatory proteins 1 and the 
      mechanisms involved.
PG  - 120-8
LID - 10.1002/iub.419 [doi]
AB  - Iron is essential for many biological processes, including oxygen delivery, and 
      its supply is tightly regulated. Iron regulatory proteins (IRPs, IRP1 and IRP2) 
      are master regulators of cellular iron metabolism. Hypoxia triggers a broad range 
      of gene responses that are primarily mediated by hypoxia-inducible factor-1 
      (HIF-1). In this study, we have shown that hypoxia could not only upregulate the 
      expression of hypoxia inducible factor-1 but also downregulate the expression of 
      IRP1. However, the molecular mechanisms that govern the IRP1 response to hypoxia 
      are not known. Herein we suggested that HIF/HRE system was an essential link 
      between IRP1 and hypoxia. The HRE of IRP1 5'-regulation regions could combine 
      with HIF-1 in vitro. Dual-luciferase reporter assay showed that IRP1 was directly 
      downregulated by HIF/HRE system.
CI  - Copyright (c) 2011 Wiley Periodicals, Inc.
FAU - Luo, Qian-Qian
AU  - Luo QQ
AD  - Department of Biochemistry, Institute of Nautical Medicine, Nantong University, 
      Nantong 226001, People's Republic of China.
FAU - Wang, Dan
AU  - Wang D
FAU - Yu, Min-Yan
AU  - Yu MY
FAU - Zhu, Li
AU  - Zhu L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - IUBMB Life
JT  - IUBMB life
JID - 100888706
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - E1UOL152H7 (Iron)
RN  - EC 1.13.12.- (Luciferases)
RN  - EC 4.2.1.3 (Iron Regulatory Protein 1)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - 5' Flanking Region
MH  - Base Sequence
MH  - Binding Sites/genetics
MH  - Cell Hypoxia/genetics
MH  - Down-Regulation
MH  - *Gene Expression Regulation
MH  - Genes, Reporter
MH  - Hep G2 Cells
MH  - Humans
MH  - Hypoxia/genetics/*metabolism
MH  - Hypoxia-Inducible Factor 1/genetics/*metabolism
MH  - Iron/metabolism
MH  - Iron Regulatory Protein 1/genetics/*metabolism
MH  - Luciferases/metabolism
MH  - Molecular Sequence Data
MH  - Oxygen/metabolism
MH  - Protein Binding/genetics
MH  - Response Elements/genetics
MH  - Up-Regulation
EDAT- 2011/03/02 06:00
MHDA- 2011/06/28 06:00
CRDT- 2011/03/02 06:00
PHST- 2011/03/02 06:00 [entrez]
PHST- 2011/03/02 06:00 [pubmed]
PHST- 2011/06/28 06:00 [medline]
AID - 10.1002/iub.419 [doi]
PST - ppublish
SO  - IUBMB Life. 2011 Feb;63(2):120-8. doi: 10.1002/iub.419.