PMID- 21360755
OWN - NLM
STAT- MEDLINE
DCOM- 20110725
LR  - 20110317
IS  - 1098-1136 (Electronic)
IS  - 0894-1491 (Linking)
VI  - 59
IP  - 5
DP  - 2011 May
TI  - Trafficking and secretion of matrix metalloproteinase-2 in olfactory ensheathing 
      glial cells: A role in cell migration?
PG  - 750-70
LID - 10.1002/glia.21146 [doi]
AB  - Olfactory ensheathing cells (OECs) are unique glia found only in the olfactory 
      system. They retain exceptional plasticity and support olfactory neurogenesis and 
      retargeting across the PNS:CNS boundary in the olfactory system. OECs have been 
      shown to improve functional outcome when transplanted into rodents with spinal 
      cord injury. The growth-promoting properties of implanted OECs encompass their 
      ability to migrate through the scar tissue and render it more permissive for 
      axonal outgrowth, but the underlying molecular mechanisms remain poorly 
      understood. OECs appear to regulate molecules of the extracellular matrix (ECM) 
      that inhibit axonal growth. Among the proteins that have the potential to promote 
      cell migration, axonal regeneration and remodeling of the ECM are matrix 
      metalloproteinases (MMPs), a family of endopeptidases that cleave matrix, 
      soluble, and membrane-bound proteins and that are regulated by their endogenous 
      inhibitors, the tissue inhibitors of MMPs (TIMPs). Little is known about MMP/TIMP 
      trafficking, secretion, and role in OECs. Using a combination of cell biology, 
      biochemistry, pharmacology, and imaging techniques, we show that MMP-2 and MMP-9 
      are expressed and proteolytically active in the olfactory epithelium and in 
      particular in the OECs of the lamina propria. These proteinases and regulatory 
      proteins such as MT1-MMP and TIMP-2 are expressed in cultured OECs. MMPs exhibit 
      nuclear localization and vesicular trafficking and secretion, with distribution 
      along microtubules and microfilaments and co-localization with the molecular 
      motor protein kinesin. Finally, we show that MMPs are involved in migration of 
      OECs in vitro on different ECM substrates.
CI  - Copyright (c) 2011 Wiley-Liss, Inc.
FAU - Gueye, Yatma
AU  - Gueye Y
AD  - Neurobiologie des Interactions Cellulaires et Neurophysiopathologie, UMR 6184 
      CNRS-Universite Aix-Marseille II, Faculte de Medecine, CS80011, Bd Pierre 
      Dramard, 13344 Marseille Cedex 15, France.
FAU - Ferhat, Lotfi
AU  - Ferhat L
FAU - Sbai, Oualid
AU  - Sbai O
FAU - Bianco, John
AU  - Bianco J
FAU - Ould-Yahoui, Adlane
AU  - Ould-Yahoui A
FAU - Bernard, Anne
AU  - Bernard A
FAU - Charrat, Eliane
AU  - Charrat E
FAU - Chauvin, Jean-Paul
AU  - Chauvin JP
FAU - Risso, Jean-Jacques
AU  - Risso JJ
FAU - Feron, Francois
AU  - Feron F
FAU - Rivera, Santiago
AU  - Rivera S
FAU - Khrestchatisky, Michel
AU  - Khrestchatisky M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110228
PL  - United States
TA  - Glia
JT  - Glia
JID - 8806785
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cell Movement/*physiology
MH  - Cells, Cultured
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Immunohistochemistry
MH  - Matrix Metalloproteinase 2/*metabolism
MH  - Matrix Metalloproteinase 9/metabolism
MH  - Neuroglia/*metabolism
MH  - Olfactory Mucosa/cytology/*metabolism
MH  - Protein Transport/physiology
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tissue Inhibitor of Metalloproteinase-2/metabolism
EDAT- 2011/03/02 06:00
MHDA- 2011/07/26 06:00
CRDT- 2011/03/02 06:00
PHST- 2010/07/30 00:00 [received]
PHST- 2010/12/20 00:00 [accepted]
PHST- 2011/03/02 06:00 [entrez]
PHST- 2011/03/02 06:00 [pubmed]
PHST- 2011/07/26 06:00 [medline]
AID - 10.1002/glia.21146 [doi]
PST - ppublish
SO  - Glia. 2011 May;59(5):750-70. doi: 10.1002/glia.21146. Epub 2011 Feb 28.