PMID- 21362221
OWN - NLM
STAT- MEDLINE
DCOM- 20121217
LR  - 20161018
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 19
IP  - 1
DP  - 2011 Feb
TI  - [Influence of immunomagnetic sorting on detecting genetic aberration of multiple 
      myeloma cells by interphase fluorescence in situ hybridization].
PG  - 54-8
AB  - This study was to aimed investigate the influence of immunomagnetic sorting on 
      detecting the genetic aberrations of multiple myeloma (MM) by interphase 
      fluorescence in situ hybridization (FISH) and to explore the detection method 
      suitable to use in our country. The genetic aberrations of immunomagnetically 
      sorted and unsorted bone marrow cells from the same MM patients were detected by 
      interphase FISH and the detectable rate of genetic aberration was compared. The 
      types of probes included 13 q14 (RB-1) and 14q32 (IGH). The 42 and 22 sorted and 
      unsorted marrow samples from MM patients were detected by using 13q14 probe and 
      14q32 probes respectively, the results indicated that the 13q14 deletion was 
      found in 9 of 42 (21.4%) unsorted marrow samples and in 25 of 42 (56.8%) 
      CD138(+)-sorted marrow samples. The 13q32 rearrangement was found in 7 of 22 
      (31.8%) unsorted marrow samples and in 14 of 22(63.6%) CD138(+)-sorted marrow 
      samples. Both of the difference was statistically significant (p = 0.001 and p = 
      0.035 respectively). Percentages of cytogenetic alterations detected in unsorted 
      bone marrow cells correlated positively with percentage of plasma cells tested by 
      bone marrow smears or flow cytometry. When percentage of plasma cells tested by 
      bone marrow smears exceed 50%, or by flow cytometry exceed 10%, there was no 
      difference between 2 methods. It is concluded that immunomagnetic sorting of 
      CD138(+) cells increases the probability of detection of the 13q14 deletion and 
      14q32 rearrangement in bone marrow samples. The low detectable rate of genetic 
      aberration in unsorted bone marrow cells is associated to the low percentage of 
      plasma cells in bone marrow samples, higher percentage of plasma cells can partly 
      overcome the shortage of unsorted detection method. When percentage of plasma 
      cells tested by bone marrow smears exceed 50%, or by flow cytometry exceed 10%, 
      there was no difference between 2 methods.
FAU - An, Gang
AU  - An G
AD  - Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical 
      Sciences, Tianjin 300020, China.
FAU - Li, Cheng-Wen
AU  - Li CW
FAU - Li, Qian
AU  - Li Q
FAU - Yi, Shu-Hua
AU  - Yi SH
FAU - Liu, Xu-Ping
AU  - Liu XP
FAU - Xu, Yan
AU  - Xu Y
FAU - Li, Zeng-Jun
AU  - Li ZJ
FAU - Qi, Jun-Yuan
AU  - Qi JY
FAU - Zou, De-Hui
AU  - Zou DH
FAU - Qiu, Lu-Gui
AU  - Qiu LG
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
SB  - IM
MH  - Cytogenetic Analysis/methods
MH  - Female
MH  - Humans
MH  - *Immunomagnetic Separation
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/diagnosis/*genetics
EDAT- 2011/03/03 06:00
MHDA- 2012/12/18 06:00
CRDT- 2011/03/03 06:00
PHST- 2011/03/03 06:00 [entrez]
PHST- 2011/03/03 06:00 [pubmed]
PHST- 2012/12/18 06:00 [medline]
AID - 1009-2137(2011)01-0054-05 [pii]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2011 Feb;19(1):54-8.