PMID- 21363889
OWN - NLM
STAT- MEDLINE
DCOM- 20110719
LR  - 20211203
IS  - 1477-9137 (Electronic)
IS  - 0021-9533 (Linking)
VI  - 124
IP  - Pt 7
DP  - 2011 Apr 1
TI  - Differential roles of kinesin and dynein in translocation of neurofilaments into 
      axonal neurites.
PG  - 1022-31
LID - 10.1242/jcs.079046 [doi]
AB  - Neurofilament (NF) subunits translocate within axons as short NFs, 
      non-filamentous punctate structures ('puncta') and diffuse material that might 
      comprise individual subunits and/or oligomers. Transport of NFs into and along 
      axons is mediated by the microtubule (MT) motor proteins kinesin and dynein. 
      Despite being characterized as a retrograde motor, dynein nevertheless 
      participates in anterograde NF transport through associating with long MTs or the 
      actin cortex through its cargo domain; relatively shorter MTs associated with the 
      motor domain are then propelled in an anterograde direction, along with any 
      linked NFs. Here, we show that inhibition of dynein function, through dynamitin 
      overexpression or intracellular delivery of anti-dynein antibody, selectively 
      reduced delivery of GFP-tagged short NFs into the axonal hillock, with a 
      corresponding increase in the delivery of puncta, suggesting that dynein 
      selectively delivered short NFs into axonal neurites. Nocodazole-mediated 
      depletion of short MTs had the same effect. By contrast, intracellular delivery 
      of anti-kinesin antibody inhibited anterograde transport of short NFs and puncta 
      to an equal extent. These findings suggest that anterograde axonal transport of 
      linear NFs is more dependent upon association with translocating MTs (which are 
      themselves translocated by dynein) than is transport of NF puncta or oligomers.
FAU - Lee, Sangmook
AU  - Lee S
AD  - Center for Cellular Neurobiology and Neurodegeneration Research, Department of 
      Biological Sciences, University of Massachusetts, One University Avenue, Lowell, 
      MA 01854, USA.
FAU - Sunil, Neethu
AU  - Sunil N
FAU - Tejada, Jennifer Motil
AU  - Tejada JM
FAU - Shea, Thomas B
AU  - Shea TB
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20110301
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Neurofilament Proteins)
RN  - EC 3.6.4.2 (Dyneins)
RN  - EC 3.6.4.4 (Kinesins)
SB  - IM
MH  - Animals
MH  - Axonal Transport
MH  - Cell Line
MH  - Dyneins/genetics/*metabolism
MH  - Kinesins/genetics/*metabolism
MH  - Mice
MH  - Microtubules/metabolism
MH  - Neurites/*metabolism
MH  - Neurofilament Proteins/genetics/*metabolism
MH  - Protein Transport
EDAT- 2011/03/03 06:00
MHDA- 2011/07/20 06:00
CRDT- 2011/03/03 06:00
PHST- 2011/03/03 06:00 [entrez]
PHST- 2011/03/03 06:00 [pubmed]
PHST- 2011/07/20 06:00 [medline]
AID - jcs.079046 [pii]
AID - 10.1242/jcs.079046 [doi]
PST - ppublish
SO  - J Cell Sci. 2011 Apr 1;124(Pt 7):1022-31. doi: 10.1242/jcs.079046. Epub 2011 Mar 
      1.