PMID- 21364122
OWN - NLM
STAT- MEDLINE
DCOM- 20110729
LR  - 20211203
IS  - 1522-1555 (Electronic)
IS  - 0193-1849 (Linking)
VI  - 300
IP  - 6
DP  - 2011 Jun
TI  - Impact of protein coingestion on muscle protein synthesis during continuous 
      endurance type exercise.
PG  - E945-54
LID - 10.1152/ajpendo.00446.2010 [doi]
AB  - This study investigates the impact of protein coingestion with carbohydrate on 
      muscle protein synthesis during endurance type exercise. Twelve healthy male 
      cyclists were studied during 2 h of fasted rest followed by 2 h of continuous 
      cycling at 55% W(max). During exercise, subjects received either 1.0 
      g.kg(-1).h(-1) carbohydrate (CHO) or 0.8 g.kg(-1).h(-1) carbohydrate with 0.2 
      g.kg(-1).h(-1) protein hydrolysate (CHO+PRO). Continuous intravenous infusions 
      with l-[ring-(13)C(6)]phenylalanine and l-[ring-(2)H(2)]tyrosine were applied, 
      and blood and muscle biopsies were collected to assess whole body protein 
      turnover and muscle protein synthesis rates at rest and during exercise 
      conditions. Protein coingestion stimulated whole body protein synthesis and 
      oxidation rates during exercise by 22 +/- 3 and 70 +/- 17%, respectively (P < 0.01). 
      Whole body protein breakdown rates did not differ between experiments. As a 
      consequence, whole body net protein balance was slightly negative in CHO and 
      positive in the CHO+PRO treatment (-4.9 +/- 0.3 vs. 8.0 +/- 0.3 mumol 
      Phe.kg(-1).h(-1), respectively, P < 0.01). Mixed muscle protein fractional 
      synthetic rates (FSR) were higher during exercise compared with resting 
      conditions (0.058 +/- 0.006 vs. 0.035 +/- 0.006%/h in CHO and 0.070 +/- 0.011 vs. 0.038 
      +/- 0.005%/h in the CHO+PRO treatment, respectively, P < 0.05). FSR during exercise 
      did not differ between experiments (P = 0.46). We conclude that muscle protein 
      synthesis is stimulated during continuous endurance type exercise activities when 
      carbohydrate with or without protein is ingested. Protein coingestion does not 
      further increase muscle protein synthesis rates during continuous endurance type 
      exercise.
FAU - Beelen, Milou
AU  - Beelen M
AD  - Department of Human Movement Sciences, NUTRIM School for Nutrition, Toxicology 
      and Metabolism, Maastricht University Medical Centre+, Maastricht, the 
      Netherlands.
FAU - Zorenc, Antoine
AU  - Zorenc A
FAU - Pennings, Bart
AU  - Pennings B
FAU - Senden, Joan M
AU  - Senden JM
FAU - Kuipers, Harm
AU  - Kuipers H
FAU - van Loon, Luc J C
AU  - van Loon LJ
LA  - eng
PT  - Journal Article
DEP - 20110301
PL  - United States
TA  - Am J Physiol Endocrinol Metab
JT  - American journal of physiology. Endocrinology and metabolism
JID - 100901226
RN  - 0 (Amino Acids)
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Dietary Proteins)
RN  - 0 (Muscle Proteins)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 42HK56048U (Tyrosine)
RN  - 47E5O17Y3R (Phenylalanine)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
SB  - IM
MH  - Amino Acids/blood
MH  - Beverages
MH  - Bicycling/*physiology
MH  - Biopsy
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - Diet
MH  - Dietary Carbohydrates/pharmacology
MH  - Dietary Proteins/*pharmacology
MH  - Humans
MH  - Lactic Acid/blood
MH  - Male
MH  - Muscle Proteins/*biosynthesis
MH  - Muscle, Skeletal/chemistry/metabolism
MH  - Phenylalanine/metabolism
MH  - Physical Endurance/*physiology
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - Tyrosine/metabolism
MH  - Young Adult
EDAT- 2011/03/03 06:00
MHDA- 2011/07/30 06:00
CRDT- 2011/03/03 06:00
PHST- 2011/03/03 06:00 [entrez]
PHST- 2011/03/03 06:00 [pubmed]
PHST- 2011/07/30 06:00 [medline]
AID - ajpendo.00446.2010 [pii]
AID - 10.1152/ajpendo.00446.2010 [doi]
PST - ppublish
SO  - Am J Physiol Endocrinol Metab. 2011 Jun;300(6):E945-54. doi: 
      10.1152/ajpendo.00446.2010. Epub 2011 Mar 1.