PMID- 21365762
OWN - NLM
STAT- MEDLINE
DCOM- 20110804
LR  - 20211020
IS  - 1615-9861 (Electronic)
IS  - 1615-9853 (Print)
IS  - 1615-9853 (Linking)
VI  - 11
IP  - 7
DP  - 2011 Apr
TI  - 193-nm photodissociation of singly and multiply charged peptide anions for acidic 
      proteome characterization.
PG  - 1329-34
LID - 10.1002/pmic.201000565 [doi]
AB  - 193-nm ultraviolet photodissociation (UVPD) was implemented to sequence singly 
      and multiply charged peptide anions. Upon dissociation by this method, a-/x-type, 
      followed by d and w side-chain loss ions, were the most prolific and abundant 
      sequence ions, often yielding 100% sequence coverage. The dissociation behavior 
      of singly and multiply charged anions was significantly different with higher 
      charged precursors yielding more sequence ions; however, all charge states 
      investigated (1- through 3-) produced rich diagnostic information. UVPD at 193 
       nm was also shown to successfully differentiate and pinpoint labile 
      phosphorylation modifications. The sequence ions were produced with high 
      abundances, requiring limited averaging for satisfactory spectral quality. The 
      intact, charge-reduced radical products generated by UV photoexcitation were also 
      subjected to collision-induced dissociation (termed, activated-electron 
      photodetachment dissociation (a-EPD)), but UVPD alone yielded more predictable 
      and higher abundance sequence ions. With the use of a basic (pH approximately 11.5), 
      piperidine-modified mobile phase, LC-MS/UVPD was implemented and resulted in the 
      successful analysis of mitogen-activated pathway kinases (MAPKs) using ultrafast 
      activation times (5  ns).
CI  - Copyright (c) 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Madsen, James A
AU  - Madsen JA
AD  - Department of Chemistry and Biochemistry, The University of Texas at Austin, 
      Austin, TX 78712, USA.
FAU - Kaoud, Tamer S
AU  - Kaoud TS
FAU - Dalby, Kevin N
AU  - Dalby KN
FAU - Brodbelt, Jennifer S
AU  - Brodbelt JS
LA  - eng
GR  - R01 GM059802/GM/NIGMS NIH HHS/United States
GR  - R01 GM059802-01A2/GM/NIGMS NIH HHS/United States
GR  - GM59802/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20110217
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
RN  - 0 (Acids)
RN  - 0 (Anions)
RN  - 0 (Peptides)
RN  - 0 (Phosphoproteins)
RN  - 0 (Proteome)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
SB  - IM
MH  - Acids/analysis/chemistry
MH  - Amino Acid Sequence
MH  - Animals
MH  - Anions/chemistry/metabolism
MH  - Electrons
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Mitogen-Activated Protein Kinase Kinases/*analysis/chemistry
MH  - Molecular Sequence Data
MH  - Peptides/*analysis/chemistry
MH  - Phosphoproteins/*analysis/chemistry
MH  - Phosphorylation
MH  - Photochemical Processes
MH  - Proteome/*analysis/chemistry
MH  - Proteomics/instrumentation/*methods
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Static Electricity
MH  - Tandem Mass Spectrometry
MH  - Ultraviolet Rays
PMC - PMC3108056
MID - NIHMS284931
COIS- The authors have declared no conflict of interest.
EDAT- 2011/03/03 06:00
MHDA- 2011/08/05 06:00
PMCR- 2012/04/01
CRDT- 2011/03/03 06:00
PHST- 2010/09/05 00:00 [received]
PHST- 2010/11/30 00:00 [revised]
PHST- 2011/01/07 00:00 [accepted]
PHST- 2011/03/03 06:00 [entrez]
PHST- 2011/03/03 06:00 [pubmed]
PHST- 2011/08/05 06:00 [medline]
PHST- 2012/04/01 00:00 [pmc-release]
AID - 10.1002/pmic.201000565 [doi]
PST - ppublish
SO  - Proteomics. 2011 Apr;11(7):1329-34. doi: 10.1002/pmic.201000565. Epub 2011 Feb 
      17.