PMID- 21365845
OWN - NLM
STAT- MEDLINE
DCOM- 20150607
LR  - 20181201
IS  - 1000-1182 (Print)
IS  - 1000-1182 (Linking)
VI  - 28
IP  - 6
DP  - 2010 Dec
TI  - [Observation on osteoblasts responded to fluid induced shear cultured on 
      different surfaces with scanning electron microscope].
PG  - 649-52
AB  - OBJECTIVE: To investigate morphological change of osteoblasts cultured on 
      titanium plates with different microarchitecture structure when exposured to 
      fluid shear stress. METHODS: 14 dynes x cm(-2) fluid shear stress was applied on 
      osteoblasts cultured on 3 different commercially pure titanium plates: Polished 
      treatment (PT), sandblast (SB), sandblasting and acid-base (SB-AB) surfaces. 
      Scanning electron microscope (SEM) was adopted to observe the morphological 
      changes after 0.5, 4, 7.5 h time point respectively. RESULTS: Morphologically, no 
      significant changes were observed after 0.5 h and few osteoblasts were seen after 
      7.5 h on all 3 type of different surfaces, and significant changes could only be 
      observed after 4 h. Osteoblasts were elongated and rearranged along the flow way 
      on different levels on PT surface. Shape of cells was altered, from long fusiform 
      suspending over depressed areas into polygon stretching out many synapsises 
      tightly attached to pits on SB-AB surface. Osteoblasts on SB surface displayed 
      similar change as SB-AB surface, besides, some cells were elongated along the way 
      of flow, stretching out threadlike synapsises attached to edges of pits. 
      CONCLUSION: Morphological change of osteoblast responding to fluid shear stress 
      in physiological range depends on substrate microarchitecture and varies with the 
      time of fluid shear stress application.
FAU - Li, Wen-Hui
AU  - Li WH
AD  - State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
FAU - Yang, Xiao-Yu
AU  - Yang XY
FAU - Xian, Su-Qin
AU  - Xian SQ
LA  - chi
PT  - Journal Article
PL  - China
TA  - Hua Xi Kou Qiang Yi Xue Za Zhi
JT  - Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal 
      of stomatology
JID - 9422648
RN  - D1JT611TNE (Titanium)
SB  - IM
MH  - *Microscopy, Electron, Scanning
MH  - *Osteoblasts
MH  - Stress, Mechanical
MH  - Titanium
EDAT- 2011/03/04 06:00
MHDA- 2015/06/08 06:00
CRDT- 2011/03/04 06:00
PHST- 2011/03/04 06:00 [entrez]
PHST- 2011/03/04 06:00 [pubmed]
PHST- 2015/06/08 06:00 [medline]
PST - ppublish
SO  - Hua Xi Kou Qiang Yi Xue Za Zhi. 2010 Dec;28(6):649-52.