PMID- 21366496
OWN - NLM
STAT- MEDLINE
DCOM- 20111020
LR  - 20110627
IS  - 1471-180X (Electronic)
IS  - 1471-180X (Linking)
VI  - 12
IP  - 4
DP  - 2011 Jul
TI  - Contrasting effects of cerebrospinal fluid from motor neuron disease patients on 
      the survival of primary motor neurons cultured with or without glia.
PG  - 257-63
LID - 10.3109/17482968.2011.560672 [doi]
AB  - Motor neuronal (MN) degeneration in motor neuron disease (MND) often starts 
      focally before spreading to neighbouring MN populations, suggesting soluble 
      factors may contribute to disease propagation. Whether cerebrospinal fluid (CSF) 
      from MND patients contains such factors has been difficult to prove. We aimed to 
      determine the effect of glia on the response of MNs to CSF from MND patients. 
      Primary rat spinal MNs grown in mono-culture or cocultured with glia were exposed 
      to CSF from patients (MND-CSF) or controls (Con-CSF) and survival measured by 
      cell counting. In mono-culture both MND-CSF and Con-CSF reduced MN survival with 
      MND-CSF reducing MN survival by less than Con-CSF. In coculture MN survival was 
      unchanged by exposure to MND-CSF while exposure to Con-CSF improved MN survival. 
      In separate experiments, murine MNs grown in mono-culture and stressed by growth 
      factor withdrawal were partially rescued by the application of monocyte 
      chemoattractant protein-1 (MCP-1), a trophic factor previously found to be 
      elevated in MND-CSF. Our results suggest that MND-CSF may contain factors harmful 
      to MNs as well as factors protective of MNs, the interplay of which is altered by 
      the presence of glial cells. These preliminary results further emphasize the 
      importance of MN environment to MN health.
FAU - Barber, Sian C
AU  - Barber SC
AD  - Sheffield Institute for Translational Neuroscience, University of Sheffield, UK.
FAU - Wood-Allum, Clare A
AU  - Wood-Allum CA
FAU - Sargsyan, Siranush A
AU  - Sargsyan SA
FAU - Walsh, Theresa
AU  - Walsh T
FAU - Cox, Laura E
AU  - Cox LE
FAU - Monk, Peter N
AU  - Monk PN
FAU - Shaw, Pamela J
AU  - Shaw PJ
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110303
PL  - England
TA  - Amyotroph Lateral Scler
JT  - Amyotrophic lateral sclerosis : official publication of the World Federation of 
      Neurology Research Group on Motor Neuron Diseases
JID - 101283386
RN  - 0 (Chemokine CCL2)
RN  - 0 (Culture Media)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Animals
MH  - *Cell Survival
MH  - Cells, Cultured
MH  - Chemokine CCL2/pharmacology
MH  - Coculture Techniques
MH  - Culture Media/chemistry
MH  - Female
MH  - Humans
MH  - Male
MH  - Mice
MH  - Middle Aged
MH  - Motor Neuron Disease/*cerebrospinal fluid/*pathology
MH  - Motor Neurons/cytology/drug effects/*pathology/*physiology
MH  - Neuroglia/cytology/*physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Young Adult
EDAT- 2011/03/04 06:00
MHDA- 2011/10/21 06:00
CRDT- 2011/03/04 06:00
PHST- 2011/03/04 06:00 [entrez]
PHST- 2011/03/04 06:00 [pubmed]
PHST- 2011/10/21 06:00 [medline]
AID - 10.3109/17482968.2011.560672 [doi]
PST - ppublish
SO  - Amyotroph Lateral Scler. 2011 Jul;12(4):257-63. doi: 
      10.3109/17482968.2011.560672. Epub 2011 Mar 3.