PMID- 21367572
OWN - NLM
STAT- MEDLINE
DCOM- 20111017
LR  - 20211020
IS  - 1873-6823 (Electronic)
IS  - 0741-8329 (Print)
IS  - 0741-8329 (Linking)
VI  - 45
IP  - 5
DP  - 2011 Aug
TI  - Opposite effects of acute ethanol exposure on GAP-43 and BDNF expression in the 
      hippocampus versus the cerebellum of juvenile rats.
PG  - 461-71
LID - 10.1016/j.alcohol.2010.12.004 [doi]
AB  - The adolescent brain is particularly vulnerable to the effects of alcohol, with 
      intoxications at this developmental age often producing long-lasting effects. The 
      present study addresses the effects of a single acute ethanol exposure on 
      growth-associated protein-43 (GAP-43) and brain-derived neurotrophic factor 
      (BDNF) gene expression in neurons in the cerebellum and hippocampus of adolescent 
      rats. Male postnatal day 23 (P23) Sprague-Dawley rats were exposed to ethanol 
      vapors for 2h and after a recovery period of 2h, the cerebellum and hippocampus 
      were harvested and samples were taken for blood alcohol concentration (BAC) 
      determinations. We found that this exposure resulted in a mean BAC of 174 mg/dL, 
      which resembles levels in human adolescents after binge drinking. Analyses of 
      total RNA and protein by quantitative reverse transcription PCR and western 
      blotting, respectively, revealed that this single ethanol exposure significantly 
      decreased the levels of GAP-43 mRNA and protein in the cerebellum but increased 
      the levels of mRNA and protein in the hippocampus. BDNF mRNA and protein levels 
      were also increased in the hippocampus but not in the cerebellum of these 
      animals. In situ hybridizations revealed that GAP-43 and BDNF mRNA levels were 
      primarily increased by alcohol exposure in hippocampal dentate granule cells and 
      CA3 neurons. Overall, the reported alterations in the expression of the 
      plasticity-associated genes GAP-43 and BDNF in juvenile rats are consistent with 
      the known deleterious effects of binge drinking on motor coordination and 
      cognitive function.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Kulkarny, V V
AU  - Kulkarny VV
AD  - Department of Neurosciences, University of New Mexico HSC, Albuquerque, USA.
FAU - Wiest, N E
AU  - Wiest NE
FAU - Marquez, C P
AU  - Marquez CP
FAU - Nixon, S C
AU  - Nixon SC
FAU - Valenzuela, C F
AU  - Valenzuela CF
FAU - Perrone-Bizzozero, N I
AU  - Perrone-Bizzozero NI
LA  - eng
GR  - T32 AA014127-08/AA/NIAAA NIH HHS/United States
GR  - R56 AA014973-06/AA/NIAAA NIH HHS/United States
GR  - T32 AA014127-02/AA/NIAAA NIH HHS/United States
GR  - R37 AA015614/AA/NIAAA NIH HHS/United States
GR  - R01 AA015614-04/AA/NIAAA NIH HHS/United States
GR  - T32 AA014127-09/AA/NIAAA NIH HHS/United States
GR  - T32AA011336/AA/NIAAA NIH HHS/United States
GR  - T32 AA014127-01/AA/NIAAA NIH HHS/United States
GR  - R01 AA015614-01/AA/NIAAA NIH HHS/United States
GR  - T32 AA014127-06/AA/NIAAA NIH HHS/United States
GR  - R01 AA014973/AA/NIAAA NIH HHS/United States
GR  - R01 AA015614-05/AA/NIAAA NIH HHS/United States
GR  - R01 AA014973-07/AA/NIAAA NIH HHS/United States
GR  - R01 AA014973-03/AA/NIAAA NIH HHS/United States
GR  - T32 AA014127-05/AA/NIAAA NIH HHS/United States
GR  - R01 AA014973-01/AA/NIAAA NIH HHS/United States
GR  - T32 AA014127-03/AA/NIAAA NIH HHS/United States
GR  - R01 AA014973-02/AA/NIAAA NIH HHS/United States
GR  - T32 AA014127-07/AA/NIAAA NIH HHS/United States
GR  - R01 AA015614/AA/NIAAA NIH HHS/United States
GR  - R01 AA014973-05/AA/NIAAA NIH HHS/United States
GR  - R01 AA014973-06A1/AA/NIAAA NIH HHS/United States
GR  - R01AA15614/AA/NIAAA NIH HHS/United States
GR  - R01 AA015614-02/AA/NIAAA NIH HHS/United States
GR  - T32 AA014127/AA/NIAAA NIH HHS/United States
GR  - R56 AA014973/AA/NIAAA NIH HHS/United States
GR  - R01 AA015614-03/AA/NIAAA NIH HHS/United States
GR  - T32 AA014127-04/AA/NIAAA NIH HHS/United States
GR  - R01 AA014973-04/AA/NIAAA NIH HHS/United States
GR  - R01AA14973/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20110302
PL  - United States
TA  - Alcohol
JT  - Alcohol (Fayetteville, N.Y.)
JID - 8502311
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (GAP-43 Protein)
RN  - 0 (RNA, Messenger)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis
MH  - Cerebellum/*drug effects/metabolism
MH  - Ethanol/*pharmacology
MH  - GAP-43 Protein/*biosynthesis
MH  - Hippocampus/*drug effects/metabolism
MH  - Male
MH  - Neurons/metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC3119774
MID - NIHMS269047
EDAT- 2011/03/04 06:00
MHDA- 2011/10/18 06:00
PMCR- 2012/08/01
CRDT- 2011/03/04 06:00
PHST- 2010/07/30 00:00 [received]
PHST- 2010/12/04 00:00 [revised]
PHST- 2010/12/06 00:00 [accepted]
PHST- 2011/03/04 06:00 [entrez]
PHST- 2011/03/04 06:00 [pubmed]
PHST- 2011/10/18 06:00 [medline]
PHST- 2012/08/01 00:00 [pmc-release]
AID - S0741-8329(11)00017-6 [pii]
AID - 10.1016/j.alcohol.2010.12.004 [doi]
PST - ppublish
SO  - Alcohol. 2011 Aug;45(5):461-71. doi: 10.1016/j.alcohol.2010.12.004. Epub 2011 Mar 
      2.