PMID- 21370135
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20110303
IS  - 1543-1894 (Print)
IS  - 1543-1894 (Linking)
VI  - 49
DP  - 2001
TI  - Fluorescent in situ hybridization : evaluation for ploidy and gene amplification.
PG  - 81-91
LID - 10.1385/1-59259-081-0:81 [doi]
AB  - In situ hybridization was first described in the late 1960s by Pardue and Gall 
      (1), who hybridized mouse ribosomal DNA sequences to a mouse chromosome spread. 
      The technique came into broader use with the description of DNA probes for 
      various viral sequences, and in the late 1980s with the publications of Lichter 
      and Ward (2), Pinkel et al. (3), and others (14-7) on the use of fluorescence in 
      situ hybridization (FISH) probes. To perform FISH, or an in situ hybridization 
      technique, a DNA sequence is prepared with its thymidine tagged with a compound 
      such as fluorescein (direct labeling), biotin, or digoxigenin to create a probe 
      for a given sequence located on a specific chromosome. Both the probe and the 
      fixed cellular DNA are denatured using a combination of heat and formamide, and 
      allowed to renature together. The nonspecifically bound probe is removed, and the 
      probe-cellular DNA complex is visualized directly, with a fluorochrome-labeled 
      avidin or a fluorochrome-tagged antidigoxigenin. For general reviews of the 
      technique, see refs. 8 -10.
FAU - Sheldon, S
AU  - Sheldon S
AD  - Department of Pathology, University of Michigan, Ann Arbor, MI.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Med
JT  - Methods in molecular medicine
JID - 101123138
EDAT- 2001/01/01 00:00
MHDA- 2001/01/01 00:01
CRDT- 2011/03/04 06:00
PHST- 2011/03/04 06:00 [entrez]
PHST- 2001/01/01 00:00 [pubmed]
PHST- 2001/01/01 00:01 [medline]
AID - 10.1385/1-59259-081-0:81 [doi]
PST - ppublish
SO  - Methods Mol Med. 2001;49:81-91. doi: 10.1385/1-59259-081-0:81.