PMID- 21371688
OWN - NLM
STAT- MEDLINE
DCOM- 20110922
LR  - 20151119
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 56
IP  - 5
DP  - 2011 May
TI  - Anti-M(3) muscarinic cholinergic autoantibodies from patients with primary 
      Sjogren's syndrome trigger production of matrix metalloproteinase-3 (MMP-3) and 
      prostaglandin E(2) (PGE(2)) from the submandibular glands.
PG  - 413-20
LID - 10.1016/j.archoralbio.2010.08.017 [doi]
AB  - BACKGROUND: We demonstrated that serum immunoglobulin G (IgG) from patients with 
      primary Sjogren's syndrome (pSS), interacting with the second extracellular loop 
      of human glandular M(3) muscarinic acetylcholine receptors (M(3) mAChR), trigger 
      the production of matrix metalloproteinase-3 (MMP-3) and prostaglandin E(2) 
      (PGE(2)). METHODS: Enzyme-linked immunosorbent assays (ELISAs) were performed in 
      the presence of M(3) mAChR synthetic peptide as antigen to detect in serum the 
      autoantibodies. Further, MMP-3 and PGE(2) production were determined in the 
      presence of anti-M(3) mAChR autoantibodies. RESULTS: An association was observed 
      between serum and anti-M(3) mAChR autoantibodies and serum levels of MMP-3 and 
      PGE(2) in pSS patients. Thus, we established that serum anti-M(3) mAChR 
      autoantibodies, MMP-3 and PGE(2) may be considered to be early markers of pSS 
      associated with inflammation. Affinity-purified anti-M(3) mAChR peptide IgG from 
      pSS patients, whilst stimulating salivary-gland M(3) mAChR, causes an increase in 
      the level of MMP-3 and PGE(2) as a result of the activation of phospholipase A(2) 
      (PLA(2)) and cyclooxygenase-2 (COX-2) (but not COX-1). CONCLUSIONS: These results 
      provide a novel insight into the role that cholinoceptor antibodies play in the 
      development of glandular inflammation. This is the first report showing that an 
      antibody interacting with glandular mAChR can induce the production of 
      pro-inflammatory mediators (MMP-3/PGE(2)).
CI  - Copyright (c) 2010 Elsevier Ltd. All rights reserved.
FAU - Reina, Silvia
AU  - Reina S
AD  - Pharmacology Unit, School of Dentistry, University of Buenos Aires, Argentina.
FAU - Sterin-Borda, Leonor
AU  - Sterin-Borda L
FAU - Passafaro, Daniela
AU  - Passafaro D
FAU - Borda, Enri
AU  - Borda E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110303
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Autoantibodies)
RN  - 0 (Biomarkers)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Receptors, Muscarinic)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 1.14.99.1 (PTGS2 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adult
MH  - Analysis of Variance
MH  - Autoantibodies/*immunology
MH  - Biomarkers/metabolism
MH  - Chromatography, Affinity
MH  - Cyclooxygenase 2/*immunology/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Immunoglobulin G/*immunology
MH  - Matrix Metalloproteinase 3/*immunology/metabolism
MH  - Middle Aged
MH  - Receptors, Muscarinic/*immunology
MH  - Sjogren's Syndrome/*immunology/metabolism
MH  - Submandibular Gland/*immunology/metabolism
EDAT- 2011/03/05 06:00
MHDA- 2011/09/23 06:00
CRDT- 2011/03/05 06:00
PHST- 2010/05/06 00:00 [received]
PHST- 2010/08/12 00:00 [revised]
PHST- 2010/08/24 00:00 [accepted]
PHST- 2011/03/05 06:00 [entrez]
PHST- 2011/03/05 06:00 [pubmed]
PHST- 2011/09/23 06:00 [medline]
AID - S0003-9969(10)00259-1 [pii]
AID - 10.1016/j.archoralbio.2010.08.017 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2011 May;56(5):413-20. doi: 10.1016/j.archoralbio.2010.08.017. 
      Epub 2011 Mar 3.