PMID- 21371879
OWN - NLM
STAT- MEDLINE
DCOM- 20110803
LR  - 20121115
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 26
IP  - 8
DP  - 2011 Apr 15
TI  - A simple assay to amplify the electrochemical signal by the aptamer based 
      biosensor modified with CdS hollow nanospheres.
PG  - 3531-5
LID - 10.1016/j.bios.2011.01.039 [doi]
AB  - A simple method to amplify the electrochemical signal by an aptamer with 22 bases 
      modified with CdS hollow nanospheres (CdSHNs) was described. Using the thrombin 
      as a model, the interaction between the aptamer and CdSHNs was characterized by 
      cyclic voltammetry, electrochemical impedance spectroscopy and circular dichroism 
      spectroscopy. CdSHNs promoted the electron transfer between the gold electrode 
      and K(3)[Fe(CN)(6)] and facilitated the conformation conversion of the aptamer 
      from hairpin to G-quadruplex after the aptamer interacted with thrombin. Under 
      optimal conditions, the modified electrode could be used for the determination of 
      thrombin from 0 to 33 mug mL(-1) and the sensitivity was 1.34 muA mL mug(-1)cm(-2), 
      while the linear range of the modified electrode without the immobilization of 
      CdSHNs was from 2.75 to 27.5 mug mL(-1) and the sensitivity was 0.062 muA mL 
      mug(-1)cm(-2). This constructed biosensor also had a good stability, specificity, 
      reproducibility and accuracy which could provide a promising platform for 
      fabrication of aptamer based biosensors.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Li, Yanfen
AU  - Li Y
AD  - Jiangsu Key Laboratory of Biofunctional Materials, Laboratory of 
      Electrochemistry, College of Chemistry and Materials Science, Nanjing Normal 
      University, Nanjing 210097, PR China.
FAU - Bao, Jianchun
AU  - Bao J
FAU - Han, Min
AU  - Han M
FAU - Dai, Zhihui
AU  - Dai Z
FAU - Wang, Huaisheng
AU  - Wang H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110302
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Cadmium Compounds)
RN  - 0 (Sulfides)
RN  - 057EZR4Z7Q (cadmium sulfide)
RN  - EC 3.4.21.5 (Thrombin)
SB  - IM
MH  - Aptamers, Nucleotide/*chemistry
MH  - Biosensing Techniques/*methods
MH  - Cadmium Compounds/*chemistry
MH  - Circular Dichroism
MH  - Electrochemical Techniques/*methods
MH  - Humans
MH  - Nanospheres/*chemistry
MH  - Reproducibility of Results
MH  - Sulfides/*chemistry
MH  - Thrombin/*analysis
EDAT- 2011/03/05 06:00
MHDA- 2011/08/04 06:00
CRDT- 2011/03/05 06:00
PHST- 2010/11/29 00:00 [received]
PHST- 2011/01/27 00:00 [revised]
PHST- 2011/01/31 00:00 [accepted]
PHST- 2011/03/05 06:00 [entrez]
PHST- 2011/03/05 06:00 [pubmed]
PHST- 2011/08/04 06:00 [medline]
AID - S0956-5663(11)00063-7 [pii]
AID - 10.1016/j.bios.2011.01.039 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2011 Apr 15;26(8):3531-5. doi: 10.1016/j.bios.2011.01.039. 
      Epub 2011 Mar 2.