PMID- 2137562
OWN - NLM
STAT- MEDLINE
DCOM- 19900322
LR  - 20181130
IS  - 0028-3835 (Print)
IS  - 0028-3835 (Linking)
VI  - 51
IP  - 1
DP  - 1990 Jan
TI  - Effects of pituitary beta-endorphin secretagogues on the concentration of 
      beta-endorphin in rat cerebrospinal fluid: evidence for a role of vasopressin in 
      the regulation of brain beta-endorphin release.
PG  - 104-10
AB  - The concentration of beta-endorphin-immunoreactivity (beta E-IR) in cerebrospinal 
      fluid (CSF) and plasma of rats was determined following intracerebroventricular 
      (ICV) treatment of conscious animals with substances known to stimulate the 
      release of beta E and other pro-opiomelanocortin (POMC)-derived peptides at the 
      level of the anterior and intermediate lobes of the pituitary. The 
      beta-adrenoceptor agoinst isoproterenol (ISO) did not influence the concentration 
      of beta E-IR in CSF collected 5-60 min after ICV administration of doses ranging 
      from 3 to 30,000 pg/rat. Plasma beta E-IR levels, however, were significantly 
      increased 20 min following ICV injection of 30,000 pg ISO. ICV treatment of 
      animals with ovine corticotropin-releasing factor (CRF; 30-30,000 pg/rat) also 
      did not affect CSF levels of beta E-IR, whereas CRF in a dose of 30 pg 
      significantly decreased, and in doses of 300-30,000 pg enhanced plasma beta E-IR 
      concentrations as determined by 20 min following treatments. ICV injection of 
      arginine8-vasopressin (AVP) in doses of 10-1,000 pg/rat dose-dependently elevated 
      the beta E-IR concentration in CSF without affecting plasma beta E-IR levels. 
      This AVP-induced increase in CSF beta E-IR was maximal 20-35 min and beta E-IR 
      levels had returned to basal 60 min following treatment. The data indicate that 
      AVP and not ISO and CRF is a stimulator of CSF levels of beta E-IR. As beta E-IR 
      in CSF likely originates from brain POMC neurons, these results suggest the hot 
      vasopressin may be a physiological regulator of brain POMC activity, and may act 
      as a releasing factor for POMC-derived peptides in the brain.
FAU - Barna, I
AU  - Barna I
AD  - Rudolf Magnus Institute, Department of Pharmacology, Medical Faculty, University 
      of Utrecht, The Netherlands.
FAU - Sweep, C G
AU  - Sweep CG
FAU - Veldhuis, H D
AU  - Veldhuis HD
FAU - Wiegant, V M
AU  - Wiegant VM
FAU - De Wied, D
AU  - De Wied D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Neuroendocrinology
JT  - Neuroendocrinology
JID - 0035665
RN  - 113-79-1 (Arginine Vasopressin)
RN  - 60617-12-1 (beta-Endorphin)
RN  - 9015-71-8 (Corticotropin-Releasing Hormone)
RN  - L628TT009W (Isoproterenol)
SB  - IM
MH  - Animals
MH  - Arginine Vasopressin/administration & dosage/*physiology
MH  - Brain/*metabolism
MH  - Corticotropin-Releasing Hormone/administration & dosage/*physiology
MH  - Injections, Intraventricular
MH  - Isoproterenol/administration & dosage/*pharmacology
MH  - Male
MH  - Pituitary Gland/metabolism
MH  - Radioimmunoassay
MH  - Rats
MH  - Rats, Inbred Strains
MH  - beta-Endorphin/blood/*cerebrospinal fluid
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - 10.1159/000125324 [doi]
PST - ppublish
SO  - Neuroendocrinology. 1990 Jan;51(1):104-10. doi: 10.1159/000125324.