PMID- 21375752
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 1743-7075 (Electronic)
IS  - 1743-7075 (Linking)
VI  - 8
IP  - 1
DP  - 2011 Mar 4
TI  - Increased ratio of dietary carbohydrate to protein shifts the focus of metabolic 
      signaling from skeletal muscle to adipose.
PG  - 13
LID - 10.1186/1743-7075-8-13 [doi]
AB  - BACKGROUND: The Dietary Reference Intakes (DRI) established acceptable 
      macronutrient distribution ranges (AMDR) for carbohydrates and protein, however 
      little is known about differences in glycemic regulations and metabolic signaling 
      across this range. This study examined metabolic outcomes associated with intake 
      of two diets differing in carbohydrate:protein ratios representing the upper and 
      lower ends of the AMDR. METHODS: Adult, male rats were fed either a high 
      carbohydrate (CHO) diet (60% of energy from carbohydrates, 12% protein, 28% fat; 
      n = 30) or a high protein (PRO) diet (35% carbohydrate, 35% protein, 30% fat; n = 
      30). Rats were meal-fed 3x/d the respective diets for 10 d and then terminated 
      after overnight food deprivation or 30, 60, 90, 120 min post-prandial (PP). 
      Plasma was collected at each of these points to provide a time course for 
      glucose, insulin and C-peptide. Skeletal muscle and adipose tissues were 
      collected at 0, 30 and 90 min for measurements of basal, early and delayed 
      activation of Akt, p70S6K and Erk 1/2. Data were analyzed by two-way ANOVA. 
      RESULTS: The CHO group produced a consistently elevated response in plasma 
      glucose, insulin and C-peptide following the meal through the 120 min time 
      course. In addition, Akt and Erk 1/2 activation in adipose was much higher than 
      in skeletal muscle. Conversely, the PRO group PP glucose response was minimal and 
      insulin maintained a response similar to a biphasic pattern. Tissue responses for 
      the PRO group were greater for Akt and p70S6K signaling in skeletal muscle 
      compared with adipose. CONCLUSION: Taken together these data suggest that 
      altering CHO:PRO ratios within the AMDR produce different glycemic response 
      patterns accompanied by differential metabolic signaling in skeletal muscle and 
      adipose.
FAU - Devkota, Suzanne
AU  - Devkota S
AD  - Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801, USA. 
      dlayman@illinois.edu.
FAU - Layman, Donald K
AU  - Layman DK
LA  - eng
PT  - Journal Article
DEP - 20110304
PL  - England
TA  - Nutr Metab (Lond)
JT  - Nutrition & metabolism
JID - 101231644
PMC - PMC3059285
EDAT- 2011/03/08 06:00
MHDA- 2011/03/08 06:01
PMCR- 2011/03/04
CRDT- 2011/03/08 06:00
PHST- 2010/09/22 00:00 [received]
PHST- 2011/03/04 00:00 [accepted]
PHST- 2011/03/08 06:00 [entrez]
PHST- 2011/03/08 06:00 [pubmed]
PHST- 2011/03/08 06:01 [medline]
PHST- 2011/03/04 00:00 [pmc-release]
AID - 1743-7075-8-13 [pii]
AID - 10.1186/1743-7075-8-13 [doi]
PST - epublish
SO  - Nutr Metab (Lond). 2011 Mar 4;8(1):13. doi: 10.1186/1743-7075-8-13.