PMID- 21375803
OWN - NLM
STAT- MEDLINE
DCOM- 20130619
LR  - 20220321
IS  - 1555-3892 (Electronic)
IS  - 0963-6897 (Linking)
VI  - 20
IP  - 11-12
DP  - 2011
TI  - Comparison of canine umbilical cord blood-derived mesenchymal stem cell 
      transplantation times: involvement of astrogliosis, inflammation, intracellular 
      actin cytoskeleton pathways, and neurotrophin-3.
PG  - 1867-80
LID - 10.3727/096368911X566163 [doi]
AB  - Canine mesenchymal stem cells (cMSCs) derived from umbilical cord blood represent 
      a potentially useful source of stem cells for therapy. The aim of this study was 
      to compare the effects of different transplantation times of cMSCs after spinal 
      cord injury (SCI). A total of 21 dogs were subjected to SCI by balloon-induced 
      compression of the first lumbar vertebrae for 12 h. Of the 21 dogs, 12 were 
      divided into four groups of three according to the time of stem cell (1 x 10(6)) 
      transplantation at the injury site: control no treatment, 12 h, 1 week, and 2 
      weeks. The remaining 9 animals were negative harvest (HA) time controls for each 
      treatment group (n = 3). Olby and Tarlov scores were used to evaluate functional 
      recovery of the hindlimbs. Markers for neuronal regeneration (Tuj-1, nestin, 
      MAP2, and NF-M), astrogliosis (GALC, GFAP, and pSTAT3), signal molecules for 
      actin cytoskeleton (RhoA, Cdc42, and Rac1), inflammation (COX-2), and 
      neurotrophins (NT-3) were evaluated by Western blot analysis. Scores of the 
      1-week transplantation group showed significant improvement compared to controls. 
      Hematoxylin and eosin (H&E) staining revealed less fibrosis at the injury site in 
      the 1-week transplantation group compared to other groups and 
      immunohistochemistry showed increased expression of neuronal markers. 
      Furthermore, in both 1-week and 2-week transplantation groups, Tuj-1, nestin, 
      MAP2, NF-M, NT-3, and GFAP increased, but pSTAT3, GALC, and COX2 decreased. RhoA 
      decreased and Rac1 and Cdc42 increased in the 1-week transplantation group. In 
      conclusion, transplantation of cMSCs 1 week after SCI was more effective in 
      improving clinical signs and neuronal regeneration and reducing fibrosis 
      formation compared to the other transplantation times evaluated. Subsequently, 
      these data may contribute to the optimization of timing for MSC transplantation 
      used as a therapeutic modality.
FAU - Park, Sung-Su
AU  - Park SS
AD  - Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National 
      University, Seoul, Korea.
FAU - Byeon, Ye-Eun
AU  - Byeon YE
FAU - Ryu, Hak-Hyun
AU  - Ryu HH
FAU - Kang, Byung-Jae
AU  - Kang BJ
FAU - Kim, YongSun
AU  - Kim Y
FAU - Kim, Wan-Hee
AU  - Kim WH
FAU - Kang, Kyung-Sun
AU  - Kang KS
FAU - Han, Ho-Jae
AU  - Han HJ
FAU - Kweon, Oh-Kyeong
AU  - Kweon OK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110304
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (Neurotrophin 3)
SB  - IM
MH  - Actin Cytoskeleton/*metabolism
MH  - Animals
MH  - Behavior, Animal/physiology
MH  - Cells, Cultured
MH  - Dogs
MH  - Female
MH  - Fetal Blood/cytology
MH  - Immunohistochemistry
MH  - Inflammation/*metabolism/veterinary
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology
MH  - Neurons/metabolism
MH  - Neurotrophin 3/*metabolism
MH  - Regeneration
MH  - Spinal Cord Injuries/pathology/therapy/veterinary
MH  - Time Factors
EDAT- 2011/03/08 06:00
MHDA- 2013/06/20 06:00
CRDT- 2011/03/08 06:00
PHST- 2011/03/08 06:00 [entrez]
PHST- 2011/03/08 06:00 [pubmed]
PHST- 2013/06/20 06:00 [medline]
AID - ct0103parketalpdf1 [pii]
AID - 10.3727/096368911X566163 [doi]
PST - ppublish
SO  - Cell Transplant. 2011;20(11-12):1867-80. doi: 10.3727/096368911X566163. Epub 2011 
      Mar 4.