PMID- 21375935
OWN - NLM
STAT- MEDLINE
DCOM- 20130124
LR  - 20110307
IS  - 1000-503X (Print)
IS  - 1000-503X (Linking)
VI  - 33
IP  - 1
DP  - 2011 Feb
TI  - Structural and functional analysis of the N-terminal region of death receptor 5.
PG  - 33-8
LID - 10.3881/j.issn.1000-503X.2011.01.008 [doi]
AB  - OBJECTIVE: To investigate the structure and function of the N-terminal region 
      (NTR) of death receptor 5 (DR5). METHODS: A series of deletions of the DR5 
      extracellular domain (DR5-ECD) proteins were expressed in E.coli. and purified by 
      affinity chromatography. The binding ability of these deletant proteins to 
      AD5-10, a mouse anti-human DR5 monoclonal antibody, was evaluated by 
      immunoblotting and ELISA. RESULTS: Recombinant DR5-ECD proteins containing the 
      NTR were recognized and bound by AD5-10, while the other deletant proteins 
      without the NTR failed to interact with AD5-10. CONCLUSION: There is an AD5-10 
      targeting site in the NTR of DR5, which may play a role in developing novel 
      immunotherapies for cancers.
FAU - Feng, Zhang
AU  - Feng Z
AD  - National Laboratory of Medical Molecular Biology, Institute of Basic Medical 
      Sciences,CAMS and PUMC, Beijing 100005, China.
FAU - Cai-feng, Chen
AU  - Cai-feng C
FAU - Shi-lian, Liu
AU  - Shi-lian L
FAU - De-xian, Zheng
AU  - De-xian Z
FAU - Yan-xin, Liu
AU  - Yan-xin L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Yi Xue Ke Xue Yuan Xue Bao
JT  - Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae
JID - 8006230
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Receptors, TNF-Related Apoptosis-Inducing Ligand)
RN  - 0 (Tnfrsf10b protein, mouse)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/chemistry
MH  - Binding Sites
MH  - Gene Deletion
MH  - Genetic Engineering
MH  - Genetic Vectors
MH  - Humans
MH  - Mice
MH  - Protein Binding
MH  - Receptors, TNF-Related Apoptosis-Inducing Ligand/*chemistry/genetics/metabolism
EDAT- 2011/03/08 06:00
MHDA- 2013/01/25 06:00
CRDT- 2011/03/08 06:00
PHST- 2011/03/08 06:00 [entrez]
PHST- 2011/03/08 06:00 [pubmed]
PHST- 2013/01/25 06:00 [medline]
AID - 10.3881/j.issn.1000-503X.2011.01.008 [doi]
PST - ppublish
SO  - Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2011 Feb;33(1):33-8. doi: 
      10.3881/j.issn.1000-503X.2011.01.008.