PMID- 21377143
OWN - NLM
STAT- MEDLINE
DCOM- 20111129
LR  - 20161125
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 56
IP  - 9
DP  - 2011 Sep
TI  - Inhibitory effect of minocycline on osteoclastogenesis in mouse bone marrow 
      cells.
PG  - 924-31
LID - 10.1016/j.archoralbio.2011.02.002 [doi]
AB  - OBJECTIVE: To study the effects of minocycline hydrochloride (MINO) on the 
      formation of tartrate-resistant acid phosphatase (TRAP) staining-positive 
      multinucleated osteoclast-like cells in mouse bone marrow cells (BMCs) treated 
      with 1alpha,25(OH)(2)D(3) or soluble receptor activator of nuclear factor-kappaB ligand 
      (s-RANKL). MATERIALS AND METHODS: Mouse BMCs were cultured in alpha-modified 
      minimum essential medium containing foetal calf serum (10%) and tetracyclines 
      (2.5, 5 and 10muM), such as MINO, tetracycline hydrochloride (TC), oxytetracycline 
      hydrochloride (OXT) or doxycycline (DOXY) in the presence of 1alpha,25(OH)(2)D(3) 
      (10nM) or s-RANKL (20ng/ml) for 7 days, and the number of TRAP staining-positive 
      osteoclast-like cells was counted. In RNA isolated from BMCs treated with 
      1alpha,25(OH)(2)D(3) or s-RANKL in the presence or absence of MINO, the expressions 
      of osteoclast differentiation relating to mRNA were analysed by reverse 
      transcription-polymerase chain reaction. Cell viability was examined in mouse 
      BMCs and rabbit osteoclasts treated with MINO (0.25-20muM and 2-50muM, 
      respectively) for 24h. RESULTS: MINO, TC, OXT or DOXY inhibited 
      1alpha,25(OH)(2)D(3)-induced osteoclast-like cell formation in mouse BMCs dose 
      dependently. MINO suppressed 1alpha,25(OH)(2)D(3)-induced up-regulation of mRNA 
      expressions of TRAP, cathepsin K, carbonic anhydrase II, and calcitonin receptor, 
      but not RANKL. MINO inhibited s-RANKL-induced osteoclast-like cell formation and 
      up-regulation of mRNA expressions for nuclear factor of activated T-cells c1 
      (NFATc1), a key regulator of osteoclast differentiation; however, MINO had no 
      effects on the viability of mouse BMCs and rabbit osteoclasts. CONCLUSION: MINO 
      inhibits RANKL-induced osteoclastogenesis via down-regulation of NFATc1 mRNA 
      expression in osteoclast precursor cells.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Nagasawa, Tsuneyasu
AU  - Nagasawa T
AD  - Department of Pharmacology, School of Dentistry, Aichi-Gakuin University, 1-100 
      Kusumoto-cho, Chikusa-ku, Nagoya 464-8650, Japan.
FAU - Arai, Michitsugu
AU  - Arai M
FAU - Togari, Akifumi
AU  - Togari A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110305
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Isoenzymes)
RN  - 0 (NFATC Transcription Factors)
RN  - 0 (RANK Ligand)
RN  - EC 3.1.3.2 (Acid Phosphatase)
RN  - EC 3.1.3.2 (Acp5 protein, mouse)
RN  - EC 3.1.3.2 (Tartrate-Resistant Acid Phosphatase)
RN  - FXC9231JVH (Calcitriol)
RN  - FYY3R43WGO (Minocycline)
SB  - IM
MH  - Acid Phosphatase/metabolism
MH  - Animals
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Bone Marrow Cells/cytology/*drug effects
MH  - Calcitriol/pharmacology
MH  - Cell Differentiation/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Down-Regulation
MH  - Isoenzymes/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Minocycline/*pharmacology
MH  - NFATC Transcription Factors/*biosynthesis
MH  - Osteoclasts/cytology/*drug effects
MH  - RANK Ligand/pharmacology
MH  - Rabbits
MH  - Tartrate-Resistant Acid Phosphatase
EDAT- 2011/03/08 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/03/08 06:00
PHST- 2010/11/11 00:00 [received]
PHST- 2011/01/06 00:00 [revised]
PHST- 2011/02/02 00:00 [accepted]
PHST- 2011/03/08 06:00 [entrez]
PHST- 2011/03/08 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - S0003-9969(11)00034-3 [pii]
AID - 10.1016/j.archoralbio.2011.02.002 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2011 Sep;56(9):924-31. doi: 10.1016/j.archoralbio.2011.02.002. 
      Epub 2011 Mar 5.