PMID- 21377442
OWN - NLM
STAT- MEDLINE
DCOM- 20110708
LR  - 20161126
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1807
IP  - 5
DP  - 2011 May
TI  - Molecular environments of divinyl chlorophylls in Prochlorococcus and 
      Synechocystis: differences in fluorescence properties with chlorophyll 
      replacement.
PG  - 471-81
LID - 10.1016/j.bbabio.2011.02.011 [doi]
AB  - A marine cyanobacterium, Prochlorococcus, is a unique oxygenic photosynthetic 
      organism, which accumulates divinyl chlorophylls instead of the monovinyl 
      chlorophylls. To investigate the molecular environment of pigments after pigment 
      replacement but before optimization of the protein moiety in photosynthetic 
      organisms, we compared the fluorescence properties of the divinyl Chl 
      a-containing cyanobacteria, Prochlorococcus marinus (CCMP 1986, CCMP 2773 and 
      CCMP 1375), by a Synechocystis sp. PCC 6803 (Synechocystis) mutant in which 
      monovinyl Chl a was replaced with divinyl Chl a. P. marinus showed a single 
      fluorescence band for photosystem (PS) II at 687nm at 77K; this was accompanied 
      with change in pigment, because the Synechocystis mutant showed the identical 
      shift. No fluorescence bands corresponding to the PS II 696-nm component and PS I 
      longer-wavelength component were detected in P. marinus, although the presence of 
      the former was suggested using time-resolved fluorescence spectra. Delayed 
      fluorescence (DF) was detected at approximately 688nm with a lifetime of 
      approximately 29ns. In striking contrast, the Synechocystis mutant showed three 
      fluorescence bands at 687, 696, and 727nm, but suppressed DF. These differences 
      in fluorescence behaviors might not only reflect differences in the molecular 
      structure of pigments but also differences in molecular environments of pigments, 
      including pigment-pigment and/or pigment-protein interactions, in the antenna and 
      electron transfer systems.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Mimuro, Mamoru
AU  - Mimuro M
AD  - Graduate School of Human and Environmental Studies, Kyoto University, Kyoto 
      606-8501, Japan.
FAU - Murakami, Akio
AU  - Murakami A
FAU - Tomo, Tatsuya
AU  - Tomo T
FAU - Tsuchiya, Tohru
AU  - Tsuchiya T
FAU - Watabe, Kazuyuki
AU  - Watabe K
FAU - Yokono, Makio
AU  - Yokono M
FAU - Akimoto, Seiji
AU  - Akimoto S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110304
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Vinyl Compounds)
RN  - 0 (divinyl chlorophyll a)
RN  - 1406-65-1 (Chlorophyll)
SB  - IM
MH  - Amino Acid Sequence
MH  - Chlorophyll/*analysis
MH  - Energy Transfer
MH  - Molecular Sequence Data
MH  - Prochlorococcus/*chemistry
MH  - Spectrometry, Fluorescence
MH  - Synechocystis/*chemistry
MH  - Vinyl Compounds/*analysis
EDAT- 2011/03/08 06:00
MHDA- 2011/07/09 06:00
CRDT- 2011/03/08 06:00
PHST- 2010/08/09 00:00 [received]
PHST- 2011/02/22 00:00 [revised]
PHST- 2011/02/28 00:00 [accepted]
PHST- 2011/03/08 06:00 [entrez]
PHST- 2011/03/08 06:00 [pubmed]
PHST- 2011/07/09 06:00 [medline]
AID - S0005-2728(11)00042-9 [pii]
AID - 10.1016/j.bbabio.2011.02.011 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2011 May;1807(5):471-81. doi: 10.1016/j.bbabio.2011.02.011. 
      Epub 2011 Mar 4.