PMID- 21377497
OWN - NLM
STAT- MEDLINE
DCOM- 20110906
LR  - 20181201
IS  - 1872-7549 (Electronic)
IS  - 0166-4328 (Linking)
VI  - 221
IP  - 1
DP  - 2011 Aug 1
TI  - NMDA-complexes linked to spatial memory performance in the Barnes maze in CD1 
      mice.
PG  - 142-8
LID - 10.1016/j.bbr.2011.02.042 [doi]
AB  - The N-methyl-d-aspartic acid receptor (NMDAR) is a well-documented key element in 
      the formation of several memories including spatial, olfactory and contextual 
      memory. Although receptor subunits have been linked to memory formation, data on 
      the involvement of the NMDAR complexes is limited. In previous work CD1 mice were 
      trained in the Barnes maze, a low-stress landmaze, and yoked controls were 
      serving as controls. Hippocampal samples from this behavioural study were taken 
      for comparing NMDAR complexes. Hippocampi were taken and stored until analysis at 
      -80  degrees C. Membrane proteins were extracted from hippocampi using an 
      ultracentrifugation step and applied on Blue Native gels that in turn were used 
      for immunoblotting with antibodies against subunits NR1, NR2A and NR2B. The 
      subunit content of the complexes was determined by denaturing two-dimensional gel 
      electrophoresis and subsequent immunoblotting. An NMDAR complex with an apparent 
      molecular weight between between 146 and 242 kDa, probably representing an NR1 
      dimer was the only complex that was significantly different between trained and 
      yoked animals. A series of NMDAR complexes containing modulatory subunits NR2A or 
      NR2B or both were detected. All complexes contained the NR1 subunit. The NR1 
      dimer complex level, increased in memory formation, may be directly or indirectly 
      involved in the process of spatial memory formation in the CD1 mouse. The results 
      are enabling and challenging further NMDAR studies, both, at the pharmacological 
      and molecular level. Moreover, several NMDAR complexes in the CD1 mouse were 
      shown to be mainly heteropolymers of subunits NR1, NR2A and NR2B, although other 
      recently described subunits were not tested due to unavailability of specific 
      antibodies. Determination of native receptor complexes rather than individual 
      subunits is mandatory and provides the molecular basis for understanding 
      mechanisms of spatial memory.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Ghafari, Maryam
AU  - Ghafari M
AD  - Department of Pediatrics, Medical University of Vienna, Wahringer Gurtel 18, 
      A-1090 Vienna, Austria.
FAU - Patil, Sudarshan S
AU  - Patil SS
FAU - Hoger, Harald
AU  - Hoger H
FAU - Pollak, Arnold
AU  - Pollak A
FAU - Lubec, Gert
AU  - Lubec G
LA  - eng
PT  - Journal Article
DEP - 20110304
PL  - Netherlands
TA  - Behav Brain Res
JT  - Behavioural brain research
JID - 8004872
RN  - 0 (NMDA receptor A1)
RN  - 0 (NR2B NMDA receptor)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - VH92ICR8HX (N-methyl D-aspartate receptor subtype 2A)
SB  - IM
MH  - Animals
MH  - Animals, Outbred Strains
MH  - Hippocampus/metabolism
MH  - Male
MH  - Maze Learning/*physiology
MH  - Memory/*physiology
MH  - Mice
MH  - Receptors, N-Methyl-D-Aspartate/*metabolism
MH  - Spatial Behavior/*physiology
EDAT- 2011/03/08 06:00
MHDA- 2011/09/07 06:00
CRDT- 2011/03/08 06:00
PHST- 2010/12/23 00:00 [received]
PHST- 2011/02/22 00:00 [revised]
PHST- 2011/02/26 00:00 [accepted]
PHST- 2011/03/08 06:00 [entrez]
PHST- 2011/03/08 06:00 [pubmed]
PHST- 2011/09/07 06:00 [medline]
AID - S0166-4328(11)00158-6 [pii]
AID - 10.1016/j.bbr.2011.02.042 [doi]
PST - ppublish
SO  - Behav Brain Res. 2011 Aug 1;221(1):142-8. doi: 10.1016/j.bbr.2011.02.042. Epub 
      2011 Mar 4.