PMID- 21377821
OWN - NLM
STAT- MEDLINE
DCOM- 20110909
LR  - 20110314
IS  - 1618-0984 (Electronic)
IS  - 0723-2020 (Linking)
VI  - 34
IP  - 2
DP  - 2011 Apr
TI  - Microbial ecology of autothermal thermophilic aerobic digester (ATAD) systems for 
      treating waste activated sludge.
PG  - 127-38
LID - 10.1016/j.syapm.2010.11.017 [doi]
AB  - Despite their widespread use, our understanding of the microbial ecology of the 
      autothermal thermophilic aerobic digesters (ATAD) used to dispose of sludge from 
      wastewater treatment plants is poor. Applying both culture-dependent and 
      molecular methods to two ATAD systems in Victoria, Australia treating different 
      wastewaters revealed that their communities were highly specialized. Denaturing 
      gradient gel electrophoresis (DGGE) profiling suggested differences in their 
      population compositions and both changed over time. However, both showed low 
      level biodiversity, and contained several novel bacterial populations. 16S rRNA 
      clone library data and FISH analyses showed that Thermus thermophilus dominated 
      both communities and that of a third ATAD plant in NSW (more than 90% of the 
      total bacterial biovolume in repeated samples taken from each of the three ATAD 
      plants). Culture-dependent methods also showed Geobacillus spp. were present in 
      both Victorian communities. Nevertheless, the ecophysiology of these populations 
      and their putative roles in sludge digestion remain unclear. 
      FISH/microautoradiographic studies did not provide conclusive data elucidating 
      which substrate/s T. thermophilus might utilize in the ATAD reactors.
CI  - Crown Copyright (c) 2011. Published by Elsevier GmbH. All rights reserved.
FAU - Hayes, David
AU  - Hayes D
AD  - Biotechnology Research Centre, La Trobe University, Bendigo, VIC 3552, Australia.
FAU - Izzard, Leonard
AU  - Izzard L
FAU - Seviour, Robert
AU  - Seviour R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110305
PL  - Germany
TA  - Syst Appl Microbiol
JT  - Systematic and applied microbiology
JID - 8306133
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
SB  - IM
MH  - Actinobacteria/genetics
MH  - *Biodegradation, Environmental
MH  - *Biota
MH  - Clostridium/genetics
MH  - Denaturing Gradient Gel Electrophoresis
MH  - Geobacillus/genetics/isolation & purification
MH  - Hot Temperature
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Microbial Consortia/*genetics
MH  - Phylogeny
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sewage/*microbiology
MH  - Thermus thermophilus/genetics/isolation & purification
EDAT- 2011/03/08 06:00
MHDA- 2011/09/10 06:00
CRDT- 2011/03/08 06:00
PHST- 2010/06/03 00:00 [received]
PHST- 2010/08/13 00:00 [revised]
PHST- 2010/11/02 00:00 [accepted]
PHST- 2011/03/08 06:00 [entrez]
PHST- 2011/03/08 06:00 [pubmed]
PHST- 2011/09/10 06:00 [medline]
AID - S0723-2020(11)00011-7 [pii]
AID - 10.1016/j.syapm.2010.11.017 [doi]
PST - ppublish
SO  - Syst Appl Microbiol. 2011 Apr;34(2):127-38. doi: 10.1016/j.syapm.2010.11.017. 
      Epub 2011 Mar 5.