PMID- 2138082
OWN - NLM
STAT- MEDLINE
DCOM- 19900423
LR  - 20141120
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 20
IP  - 2
DP  - 1990 Feb
TI  - Difference in the clustering of complement receptor type 1 (CR1) on 
      polymorphonuclear leukocytes and erythrocytes: effect on immune adherence.
PG  - 283-9
AB  - Complement receptor type 1 (CR1) mediates the adherence of complement-reacted 
      immune complexes (IC) to various blood cells. On the erythrocyte, CR1 are 
      clustered, a distribution which favors efficient multivalent binding of 
      C3b-coated IC. IC can also bind to CR1 expressed on polymorphonuclear (PMN) 
      leukocytes. To evaluate the respective importance of these two cell types in 
      immune adherence reactions, functional analysis of IC binding, as well as 
      morphological studies of CR1 distribution at their surface were undertaken. At 
      equal cell concentrations, resting PMN leukocytes bound the same percentage of IC 
      as erythrocytes, despite expressing four times more CR1 at their surface. At 
      equal CR1 concentrations, IC binding to resting or 
      formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated PMN leukocytes was always 
      lower than to erythrocytes. The morphological counterpart of these differences 
      was studied by label-fracture immunoelectron microscopy. On erythrocytes, almost 
      50% of the CR1 were distributed in clusters of greater than or equal to 3 units, 
      while less than 15% were grouped in such clusters on the surface of PMN 
      leukocytes. Activation of PMN leukocytes by fMLP increased the surface density of 
      CR1, but the proportion of clustered CR1 remained unchanged. These observations 
      suggest that the low responsiveness of PMN leukocytes towards C3b-coated IC may 
      be due to the unaggregated state of CR1. In the circulation, erythrocytes might 
      function as a "buffer" for PMN leukocytes, which would otherwise engage too 
      swiftly in reactions with IC.
FAU - Paccaud, J P
AU  - Paccaud JP
AD  - Department of Medicine, Centre Medical Universitaire, Geneva, Switzerland.
FAU - Carpentier, J L
AU  - Carpentier JL
FAU - Schifferli, J A
AU  - Schifferli JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Complement C3)
RN  - 0 (Opsonin Proteins)
RN  - 0 (Receptors, Complement)
RN  - 0 (Receptors, Complement 3b)
RN  - 0 (Receptors, Fc)
RN  - 0 (Receptors, IgG)
SB  - IM
MH  - Antigen-Antibody Complex/*metabolism
MH  - Antigens, Differentiation/metabolism
MH  - Cell Membrane/ultrastructure
MH  - Complement C3/metabolism
MH  - Erythrocytes/*immunology/ultrastructure
MH  - Freeze Fracturing
MH  - Humans
MH  - Immunologic Techniques
MH  - In Vitro Techniques
MH  - Microscopy, Electron
MH  - Neutrophils/*immunology/ultrastructure
MH  - Opsonin Proteins
MH  - Receptor Aggregation
MH  - Receptors, Complement/*metabolism
MH  - Receptors, Complement 3b
MH  - Receptors, Fc/metabolism
MH  - Receptors, IgG
EDAT- 1990/02/01 00:00
MHDA- 1990/02/01 00:01
CRDT- 1990/02/01 00:00
PHST- 1990/02/01 00:00 [pubmed]
PHST- 1990/02/01 00:01 [medline]
PHST- 1990/02/01 00:00 [entrez]
AID - 10.1002/eji.1830200209 [doi]
PST - ppublish
SO  - Eur J Immunol. 1990 Feb;20(2):283-9. doi: 10.1002/eji.1830200209.