PMID- 21382392
OWN - NLM
STAT- MEDLINE
DCOM- 20111014
LR  - 20211020
IS  - 1873-507X (Electronic)
IS  - 0031-9384 (Print)
IS  - 0031-9384 (Linking)
VI  - 104
IP  - 2
DP  - 2011 Aug 3
TI  - Strain dependent effects of prenatal stress on gene expression in the rat 
      hippocampus.
PG  - 334-9
LID - 10.1016/j.physbeh.2011.02.032 [doi]
AB  - Multiple animal models have been developed to recapitulate phenotypes of the 
      human disease, schizophrenia. A model that simulates many of the cognitive and 
      sensory deficits of the disorder is the use of random variable prenatal stress 
      (PS) in the rat. These deficits suggest a molecular origin in the hippocampus, a 
      brain region that plays a role in the regulation of stress. To study both 
      hippocampal gene expression changes in offspring of prenatally stressed dams and 
      to address genetic variability, we used a random array of prenatal stressors in 
      three different rat strains with diverse responses to stress: Fischer, 
      Sprague-Dawley, and Lewis rats. Candidate genes involved in stress, 
      schizophrenia, cognition, neurotrophic effects, and immunity were selected for 
      assessment by real-time quantitative PCR under resting conditions and following a 
      brief exposure to restraint stress. PS resulted in significant differences in 
      gene expression in the offspring that were strain dependent. mRNA expression for 
      the N-methyl-D-aspartate receptor subtype 2B (Grin2b) was increased, and tumor 
      necrosis factor-alpha (Tnfalpha) transcript was decreased in PS Sprague-Dawley and 
      Lewis rats, but not in the Fischer rats. Expression of brain-derived neurotrophic 
      factor (Bdnf) mRNA in the hippocampus was increased after an acute stress in all 
      controls of each strain, yet a decrease was seen after acute stress in the PS 
      Sprague-Dawley and Lewis rats. Expression of the glucocorticoid receptor (Nr3c1) 
      was decreased in the Fischer strain when compared to Lewis or Sprague-Dawley 
      rats, though the Fischer rats had markedly higher alpha7 nicotinic receptor (Chrna7) 
      expression. The expression differences seen in these animals may be important 
      elements of the phenotypic differences seen due to PS and genetic background.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Neeley, Eric W
AU  - Neeley EW
AD  - Department of Psychiatry, University of Colorado Denver, Aurora, CO 80045, USA.
FAU - Berger, Ralph
AU  - Berger R
FAU - Koenig, James I
AU  - Koenig JI
FAU - Leonard, Sherry
AU  - Leonard S
LA  - eng
GR  - MH81177/MH/NIMH NIH HHS/United States
GR  - R01 MH081177/MH/NIMH NIH HHS/United States
GR  - R01 DA009457-13/DA/NIDA NIH HHS/United States
GR  - R01 MH081177-04/MH/NIMH NIH HHS/United States
GR  - R01 DA009457/DA/NIDA NIH HHS/United States
GR  - DA09457/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20110304
PL  - United States
TA  - Physiol Behav
JT  - Physiology & behavior
JID - 0151504
RN  - 0 (Adrenal Cortex Hormones)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Chrna7 protein, human)
RN  - 0 (Chrna7 protein, rat)
RN  - 0 (NR2B NMDA receptor)
RN  - 0 (NR3C1 protein, rat)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (Receptors, Nicotinic)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (alpha7 Nicotinic Acetylcholine Receptor)
SB  - IM
MH  - Adrenal Cortex Hormones/blood
MH  - Age Factors
MH  - Animals
MH  - Animals, Newborn
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Female
MH  - Gene Expression Regulation, Developmental/*genetics
MH  - Hippocampus/growth & development/*metabolism
MH  - Male
MH  - Pregnancy
MH  - Prenatal Exposure Delayed Effects/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Radioimmunoassay
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Rats, Sprague-Dawley
MH  - Receptors, Glucocorticoid/genetics/metabolism
MH  - Receptors, N-Methyl-D-Aspartate/genetics/metabolism
MH  - Receptors, Nicotinic/genetics/metabolism
MH  - Species Specificity
MH  - Stress, Psychological/*genetics/metabolism/*pathology
MH  - Tumor Necrosis Factor-alpha/genetics/metabolism
MH  - alpha7 Nicotinic Acetylcholine Receptor
PMC - PMC3118943
MID - NIHMS285013
EDAT- 2011/03/09 06:00
MHDA- 2011/10/15 06:00
PMCR- 2012/08/03
CRDT- 2011/03/09 06:00
PHST- 2010/10/13 00:00 [received]
PHST- 2011/01/30 00:00 [revised]
PHST- 2011/02/21 00:00 [accepted]
PHST- 2011/03/09 06:00 [entrez]
PHST- 2011/03/09 06:00 [pubmed]
PHST- 2011/10/15 06:00 [medline]
PHST- 2012/08/03 00:00 [pmc-release]
AID - S0031-9384(11)00096-5 [pii]
AID - 10.1016/j.physbeh.2011.02.032 [doi]
PST - ppublish
SO  - Physiol Behav. 2011 Aug 3;104(2):334-9. doi: 10.1016/j.physbeh.2011.02.032. Epub 
      2011 Mar 4.