PMID- 21383953
OWN - NLM
STAT- MEDLINE
DCOM- 20110608
LR  - 20211020
IS  - 1449-2288 (Electronic)
IS  - 1449-2288 (Linking)
VI  - 7
IP  - 2
DP  - 2011 Feb 14
TI  - Gamma-ray irradiation impairs dendritic cell migration to CCL19 by 
      down-regulation of CCR7 and induction of cell apoptosis.
PG  - 168-79
AB  - Dendritic cells (DCs) are the most potent antigen-presenting cells and play a 
      crucial role in the regulation of immune response and migration of DCs into 
      secondary lymphoid tissues also play an important role in the initiation of 
      innate and adaptive immunity. Radiation therapy is now a routine treatment for 
      certain types of cancer and over 20 percent of cancer patients will require 
      radiation therapy during the treatment of their disease. However, the influence 
      of ionizing irradiation on the migratory ability of DCs is largely unknown. In 
      this article, we report that gamma ray irradiation can significantly inhibit 
      LPS-triggered up regulation of CCR7 expression and PGE2 production by DC, thus 
      impairing DC migration towards CCL19 in vitro and in vivo. Moreover, gamma ray 
      exposed DC also displayed an increased apoptosis rate and decreased cell 
      viability. Furthermore, we demonstrate that exogenous PGE2 can partly reduce the 
      gamma-ray induced migratory impairment and restored CCR7 expression of DC. Our 
      work suggests that gamma irradiation affects DC function at multiple steps during the 
      immune response including DC migration, and that PGE2, via control of CCR7 
      expression, is an important regulator of DC migration.
FAU - Liu, Cong
AU  - Liu C
AD  - Department of Radiation Medicine, Faculty of Naval Medicine, Second Military 
      Medical University, Shanghai, PR China.
FAU - Lin, Jin
AU  - Lin J
FAU - Zhao, Luqian
AU  - Zhao L
FAU - Yang, Yanyong
AU  - Yang Y
FAU - Gao, Fu
AU  - Gao F
FAU - Li, Bailong
AU  - Li B
FAU - Cui, Jianguo
AU  - Cui J
FAU - Cai, Jianming
AU  - Cai J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110214
PL  - Australia
TA  - Int J Biol Sci
JT  - International journal of biological sciences
JID - 101235568
RN  - 0 (Chemokine CCL19)
RN  - 0 (Receptors, CCR7)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Cell Movement/*drug effects/*radiation effects
MH  - Cell Survival/drug effects/radiation effects
MH  - Cells, Cultured
MH  - Chemokine CCL19/*pharmacology
MH  - Dendritic Cells/*drug effects/*radiation effects
MH  - Enzyme-Linked Immunosorbent Assay
MH  - *Gamma Rays
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Receptors, CCR7/*metabolism
PMC - PMC3048846
OTO - NOTNLM
OT  - Apoptosis.
OT  - CCR7
OT  - Dendritic cell
OT  - Migration
OT  - gamma ray Irradiation
COIS- Conflict of Interests: The authors have declared that no conflict of interest 
      exists.
EDAT- 2011/03/09 06:00
MHDA- 2011/06/09 06:00
PMCR- 2011/01/01
CRDT- 2011/03/09 06:00
PHST- 2010/09/30 00:00 [received]
PHST- 2011/02/05 00:00 [accepted]
PHST- 2011/03/09 06:00 [entrez]
PHST- 2011/03/09 06:00 [pubmed]
PHST- 2011/06/09 06:00 [medline]
PHST- 2011/01/01 00:00 [pmc-release]
AID - ijbsv07p0168 [pii]
AID - 10.7150/ijbs.7.168 [doi]
PST - epublish
SO  - Int J Biol Sci. 2011 Feb 14;7(2):168-79. doi: 10.7150/ijbs.7.168.