PMID- 21384169
OWN - NLM
STAT- MEDLINE
DCOM- 20120217
LR  - 20220317
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 38
IP  - 8
DP  - 2011 Nov
TI  - MCP-1 -2518 A/G functional polymorphism is associated with increased 
      susceptibility to active pulmonary tuberculosis in Tunisian patients.
PG  - 5413-9
LID - 10.1007/s11033-011-0695-4 [doi]
AB  - Monocyte chemoattractant protein-1 (MCP-1) plays crucial role in protective 
      immunity against Mycobacterium tuberculosis (MT). In this study, we examined 
      whether single nucleotide polymorphism (SNP) -2518 A/G (rs 1024611) of MCP-1 
      affect the susceptibility to active tuberculosis (TB) in Tunisian populations. 
      Genomic DNA from patients with active TB (168 cases of pulmonary TB and 55 cases 
      of extrapulmonary TB) and ethnically controls (150 cases) was genotyped for the 
      MCP-1 -2518 A/G SNP by polymerase chain reaction fragment length polymorphism 
      (PCR-RFLP). We observed that -2518 G allele and GG genotype (high MCP-1 producer) 
      frequencies were significantly more elevated in active pulmonary TB group in 
      comparison to control group [34 vs. 22%; P = 0.0007; 15 vs. 5%, P corrected for 
      the number of genotypes (Pc) = 0.015; respectively]. Additionally, they were 
      associated with increased risk development of this clinical form of TB [odds 
      ratio (OR) = 1.83, 95% confidence intervals (CI) = 1.26-2.66; OR = 3.1, 95% CI = 
      1.28-7.76; respectively]. However, wild type allele -2518 A and AA genotype were 
      over-represented in control group (78 and 62%) and seem to be protective factors 
      against TB. Moreover, -2518 AA genotype was more frequent in control group and 
      was associated with resistance against development of active pulmonary TB (OR = 
      0.56, 95% CI = 0.35-0.89, Pc = 0.03). Our findings confirm the key role of -2518 
      A/G SNP of MCP-1 and support its association with resistance/susceptibility to 
      the development of active pulmonary TB in the Tunisian population.
FAU - Ben-Selma, Walid
AU  - Ben-Selma W
AD  - Laboratory of Microbiology and Immunology, UR02SP13, Farhat Hached University 
      Hospital, CHU Farhat Hached - Av. Ibn el Jazzar, 4000 Sousse, Tunisia. 
      wbenselma@hotmail.com
FAU - Harizi, Hedi
AU  - Harizi H
FAU - Boukadida, Jalel
AU  - Boukadida J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110308
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Alleles
MH  - Case-Control Studies
MH  - Chemokine CCL2/*genetics
MH  - Demography
MH  - Female
MH  - Gene Frequency/genetics
MH  - *Genetic Predisposition to Disease
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Restriction Fragment Length
MH  - Polymorphism, Single Nucleotide/*genetics
MH  - Tuberculosis, Pulmonary/*genetics
MH  - Tunisia
MH  - Young Adult
EDAT- 2011/03/09 06:00
MHDA- 2012/02/18 06:00
CRDT- 2011/03/09 06:00
PHST- 2010/11/02 00:00 [received]
PHST- 2011/02/26 00:00 [accepted]
PHST- 2011/03/09 06:00 [entrez]
PHST- 2011/03/09 06:00 [pubmed]
PHST- 2012/02/18 06:00 [medline]
AID - 10.1007/s11033-011-0695-4 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2011 Nov;38(8):5413-9. doi: 10.1007/s11033-011-0695-4. Epub 2011 
      Mar 8.