PMID- 21384262
OWN - NLM
STAT- MEDLINE
DCOM- 20111121
LR  - 20220408
IS  - 1432-0886 (Electronic)
IS  - 0009-5915 (Linking)
VI  - 120
IP  - 4
DP  - 2011 Aug
TI  - Homolog pairing and sister chromatid cohesion in heterochromatin in Drosophila 
      male meiosis I.
PG  - 335-51
LID - 10.1007/s00412-011-0314-0 [doi]
AB  - Drosophila males undergo meiosis without recombination or chiasmata but 
      homologous chromosomes pair and disjoin regularly. The X-Y pair utilizes a 
      specific repeated sequence within the heterochromatic ribosomal DNA blocks as a 
      pairing site. No pairing sites have yet been identified for the autosomes. To 
      search for such sites, we utilized probes targeting specific heterochromatic 
      regions to assay heterochromatin pairing sequences and behavior in meiosis by 
      fluorescence in situ hybridization (FISH). We found that the small fourth 
      chromosome pairs at heterochromatic region 61 and associates with the X 
      chromosome throughout prophase I. Homolog pairing of the fourth chromosome is 
      disrupted when the homolog conjunction complex is perturbed by mutations in SNM 
      or MNM. On the other hand, six tested heterochromatic regions of the major 
      autosomes proved to be largely unpaired after early prophase I, suggesting that 
      stable homolog pairing sites do not exist in heterochromatin of the major 
      autosomes. Furthermore, FISH analysis revealed two distinct patterns of sister 
      chromatid cohesion in heterochromatin: regions with stable cohesion and regions 
      lacking cohesion. This suggests that meiotic sister chromatid cohesion is 
      incomplete within heterochromatin and may occur at specific preferential sites.
FAU - Tsai, Jui-He
AU  - Tsai JH
AD  - Department of Biochemistry, Cellular, and Molecular Biology, University of 
      Tennessee, Knoxville, USA. jtsai1@utk.edu
FAU - Yan, Rihui
AU  - Yan R
FAU - McKee, Bruce D
AU  - McKee BD
LA  - eng
GR  - R01GM40489/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20110308
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
RN  - 0 (Heterochromatin)
RN  - 0 (Molecular Probes)
SB  - IM
MH  - Animals
MH  - Centromere/chemistry/genetics
MH  - *Chromatids/chemistry/genetics/metabolism
MH  - Chromosome Pairing
MH  - Chromosome Segregation
MH  - Drosophila melanogaster/*genetics
MH  - Fluorescence
MH  - Heterochromatin/*chemistry/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - *Meiosis
MH  - Molecular Probes/analysis
MH  - Mutation
MH  - Spermatocytes/cytology/*metabolism
MH  - X Chromosome/*chemistry/genetics
MH  - Y Chromosome/*chemistry/genetics
EDAT- 2011/03/09 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/03/09 06:00
PHST- 2010/08/16 00:00 [received]
PHST- 2011/02/13 00:00 [accepted]
PHST- 2011/02/11 00:00 [revised]
PHST- 2011/03/09 06:00 [entrez]
PHST- 2011/03/09 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - 10.1007/s00412-011-0314-0 [doi]
PST - ppublish
SO  - Chromosoma. 2011 Aug;120(4):335-51. doi: 10.1007/s00412-011-0314-0. Epub 2011 Mar 
      8.