PMID- 21385595
OWN - NLM
STAT- MEDLINE
DCOM- 20110908
LR  - 20110527
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 158
IP  - 1-2
DP  - 2011 Jun
TI  - Identification of new defective interfering RNA species associated with porcine 
      reproductive and respiratory syndrome virus infection.
PG  - 33-6
LID - 10.1016/j.virusres.2011.03.001 [doi]
AB  - In the present study, seven new defective interfering (DI) RNA species of porcine 
      reproductive and respiratory syndrome virus (PRRSV) were identified. RT-PCR, 
      Northern blot and sequence analyses indicated that these DI RNA specie have 
      deletions of 8513-9176 nucleotides located between Nsp1/Nsp2 and Nsp10. Compared 
      with the previous DI RNAs of PRRSV reported, they have three distinct 
      characteristics: much smaller deletion sizes; different nucleotide repeats 
      (2-12nt) used in the junction sites and in-frame deletions. The results further 
      suggested that the similarity-assisted RNA recombination may be the main cause of 
      generation of DI RNAs in PRRSV and probably in other arteriviruses.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Xiao, Chao-Ting
AU  - Xiao CT
AD  - College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, 
      PR China.
FAU - Liu, Zhong-Hua
AU  - Liu ZH
FAU - Yu, Xing-Long
AU  - Yu XL
FAU - Ge, Meng
AU  - Ge M
FAU - Li, Run-Cheng
AU  - Li RC
FAU - Xiao, Bo-Ren
AU  - Xiao BR
FAU - Zhou, Hong-Rui
AU  - Zhou HR
LA  - eng
SI  - GENBANK/JF430261
SI  - GENBANK/JF430262
SI  - GENBANK/JF430263
SI  - GENBANK/JF430264
SI  - GENBANK/JF430265
SI  - GENBANK/JF430266
SI  - GENBANK/JF430267
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110306
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Defective Viruses/genetics/*isolation & purification
MH  - Molecular Sequence Data
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*growth & 
      development
MH  - RNA Viruses/genetics/*isolation & purification
MH  - RNA, Viral/*genetics
MH  - Recombination, Genetic
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Sequence Deletion
EDAT- 2011/03/10 06:00
MHDA- 2011/09/09 06:00
CRDT- 2011/03/10 06:00
PHST- 2010/12/02 00:00 [received]
PHST- 2011/02/28 00:00 [revised]
PHST- 2011/03/01 00:00 [accepted]
PHST- 2011/03/10 06:00 [entrez]
PHST- 2011/03/10 06:00 [pubmed]
PHST- 2011/09/09 06:00 [medline]
AID - S0168-1702(11)00073-6 [pii]
AID - 10.1016/j.virusres.2011.03.001 [doi]
PST - ppublish
SO  - Virus Res. 2011 Jun;158(1-2):33-6. doi: 10.1016/j.virusres.2011.03.001. Epub 2011 
      Mar 6.