PMID- 21385650
OWN - NLM
STAT- MEDLINE
DCOM- 20110421
LR  - 20160518
IS  - 1875-9572 (Print)
IS  - 1875-9572 (Linking)
VI  - 52
IP  - 1
DP  - 2011 Feb
TI  - Downregulation of caveolin-1 in a murine model of acute allergic airway disease.
PG  - 5-10
LID - 10.1016/j.pedneo.2010.12.006 [doi]
AB  - BACKGROUND: Airway remodeling refers to the structural changes in the airways of 
      asthma. Caveolin-1 reduces cell growth and negatively regulates smooth muscle 
      cell proliferation. The aim was to investigate lung caveolin-1 status in a murine 
      model of acute allergic airway disease. METHODS: Six- to eight-week-old female 
      BALB/c mice were sensitized by intraperitoneal injections of phosphate-buffered 
      saline or ovalbumin (OVA) and aluminium hydroxide on Days 0 and 14, challenged 
      with aerosolized saline or OVA (1%) on Days 21-25, 28-32, and 35. The mice were 
      killed 1 day after the last OVA/saline challenge. Serum OVA-specific 
      immunoglobulin E (IgE) was measured by enzyme-linked immunosorbent assay. 
      Peribronchial inflammation was quantified by morphometric analysis. Lung 
      caveolin-1 and Type I collagen mRNA expression was determined by real-time 
      reverse-transcription polymerase chain reaction. Total lung collagen was measured 
      using Sircol Assay Kit. RESULTS: Serum OVA-specific IgE levels were significantly 
      elevated in OVA-challenged mice when compared with saline-challenged mice. 
      Percentage of inflammatory cells in the bronchoalveolar lavage was significantly 
      higher in the OVA-challenged animals. The animals' lungs that were sensitized and 
      challenged with OVA contained large numbers of inflammatory cells concentrated 
      near the airways and in the perivascular areas. The thickness of the bronchial 
      epithelial layer and smooth muscle layer and the numbers of total inflammatory 
      cells and eosinophils significantly increased in OVA-challenged mice. Caveolin-1 
      mRNA expression significantly decreased and Type I collagen mRNA expression 
      significantly increased in the lung tissue of OVA-challenged mice. CONCLUSION: 
      These results suggest that caveolin-1 seems to be involved in the pathogenesis of 
      airway remodeling of acute allergic airway disease.
CI  - Copyright (c) 2011. Published by Elsevier B.V.
FAU - Chen, Chung-Ming
AU  - Chen CM
AD  - Department of Pediatrics, Dajia Lee's Hospital, Lee's Medical Corporation, 
      Taichung, Taiwan.
FAU - Wu, Meng-Ying
AU  - Wu MY
FAU - Chou, Hsiu-Chu
AU  - Chou HC
FAU - Lang, Yaw-Dong
AU  - Lang YD
FAU - Wang, Leng-Fang
AU  - Wang LF
LA  - eng
PT  - Journal Article
DEP - 20110218
PL  - Singapore
TA  - Pediatr Neonatol
JT  - Pediatrics and neonatology
JID - 101484755
RN  - 0 (Biomarkers)
RN  - 0 (Caveolin 1)
RN  - 9007-34-5 (Collagen)
SB  - IM
CIN - Pediatr Neonatol. 2011 Feb;52(1):1-2. PMID: 21385648
MH  - Acute Disease
MH  - Airway Remodeling
MH  - Animals
MH  - Asthma/*metabolism
MH  - Biomarkers/metabolism
MH  - Bronchoalveolar Lavage
MH  - Caveolin 1/*metabolism
MH  - Collagen/metabolism
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2011/03/10 06:00
MHDA- 2011/04/22 06:00
CRDT- 2011/03/10 06:00
PHST- 2009/12/16 00:00 [received]
PHST- 2010/04/07 00:00 [revised]
PHST- 2010/04/27 00:00 [accepted]
PHST- 2011/03/10 06:00 [entrez]
PHST- 2011/03/10 06:00 [pubmed]
PHST- 2011/04/22 06:00 [medline]
AID - S1875-9572(10)00014-8 [pii]
AID - 10.1016/j.pedneo.2010.12.006 [doi]
PST - ppublish
SO  - Pediatr Neonatol. 2011 Feb;52(1):5-10. doi: 10.1016/j.pedneo.2010.12.006. Epub 
      2011 Feb 18.