PMID- 21389746
OWN - NLM
STAT- MEDLINE
DCOM- 20110805
LR  - 20110419
IS  - 1423-0313 (Electronic)
IS  - 0031-7012 (Linking)
VI  - 87
IP  - 3-4
DP  - 2011
TI  - Effects of different types of anesthetic agents on cellular protein kinase C-gamma 
      dynamics in mouse brain.
PG  - 180-6
LID - 10.1159/000324317 [doi]
AB  - BACKGROUND/AIMS: Although protein kinase C-gamma (PKC-gamma) is a target for the effects 
      of volatile anesthetics, the molecular mechanisms of the kinase function during 
      their action remain unclear. We examined the effects of different types of 
      anesthetics on PKC-gamma knockout mice. Furthermore, we investigated the dynamics of 
      the kinase in brain cells obtained from mice anesthetized with these anesthetics. 
      METHODS: We measured the required times for loss of righting reflex (rtfLORR) 
      after administration of isoflurane, sevoflurane, or propofol on PKC-gamma knockout 
      mice and compared the times with those of wild-type mice. We also used 
      immunoblotting to investigate the intracellular distribution of PKC-gamma and 
      phosphorylated PKC-gamma (p-PKC-gamma) in brain cell fractions obtained from wild-type 
      mice during the loss of righting reflex induced by these anesthetics. RESULTS: 
      Isoflurane (2.6%) and sevoflurane (3.4%) used at twice the minimum alveolar 
      concentration significantly prolonged the rtfLORRs in PKC-gamma knockout mice 
      compared to those in wild-type mice. On the other hand, no significant difference 
      was observed between knockout and wild-type mice treated with propofol (200 
      mg/kg). Examination of the cellular fractions isolated from volatile 
      anesthetic-treated mouse brains showed that PKC-gamma was significantly decreased in 
      the synaptic membrane fraction (P2), whereas p-PKC-gamma was significantly increased 
      in P2. There was no significant change in the supernatant fraction (S). In 
      propofol-treated mice, PKC-gamma and p-PKC-gamma showed no significant changes in P2 or 
      S. CONCLUSION: Our results provide new evidence to support the possibility of the 
      involvement of PKC-gamma in the actions of volatile anesthetics.
CI  - Copyright (c) 2011 S. Karger AG, Basel.
FAU - Takeda, Akiko
AU  - Takeda A
AD  - Department of Anesthesiology, Tokyo Medical University Hospital, Tokyo, Japan. 
      a-takeda @ tokyo-med.ac.jp
FAU - Miyashita, Ryoichi
AU  - Miyashita R
FAU - Nagura, Takeo
AU  - Nagura T
FAU - Sekine, Shusuke
AU  - Sekine S
FAU - Murozono, Michihiro
AU  - Murozono M
FAU - Matsumoto, Shohei
AU  - Matsumoto S
FAU - Uchino, Hiroyuki
AU  - Uchino H
LA  - eng
PT  - Journal Article
DEP - 20110310
PL  - Switzerland
TA  - Pharmacology
JT  - Pharmacology
JID - 0152016
RN  - 0 (Anesthetics, Inhalation)
RN  - 0 (Anesthetics, Intravenous)
RN  - EC 2.7.1.- (protein kinase C gamma)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Anesthetics, Inhalation/administration & dosage/*pharmacology
MH  - Anesthetics, Intravenous/administration & dosage/*pharmacology
MH  - Animals
MH  - Blotting, Western
MH  - Brain/*drug effects/enzymology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Phosphorylation
MH  - Protein Kinase C/genetics/*metabolism/physiology
MH  - Subcellular Fractions/drug effects/enzymology
EDAT- 2011/03/11 06:00
MHDA- 2011/08/06 06:00
CRDT- 2011/03/11 06:00
PHST- 2010/12/21 00:00 [received]
PHST- 2011/01/12 00:00 [accepted]
PHST- 2011/03/11 06:00 [entrez]
PHST- 2011/03/11 06:00 [pubmed]
PHST- 2011/08/06 06:00 [medline]
AID - 000324317 [pii]
AID - 10.1159/000324317 [doi]
PST - ppublish
SO  - Pharmacology. 2011;87(3-4):180-6. doi: 10.1159/000324317. Epub 2011 Mar 10.