PMID- 21389907 OWN - NLM STAT- MEDLINE DCOM- 20110616 LR - 20211020 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 16 IP - 3 DP - 2011 Mar 9 TI - Identification and determination of flavonoids in astragali radix by high performance liquid chromatography coupled with DAD and ESI-MS detection. PG - 2293-303 LID - 10.3390/molecules16032293 [doi] AB - A method for the analysis of flavonoids in Astragali Radix by high-performance liquid chromatography (HPLC) combined with photodiode-array detection (DAD) and an electrospray ionization (ESI)--mass spectrometry (MS) was developed. After the samples were extracted with ethanol, the optimum separation conditions for these analytes were achieved using a gradient elution system and a 2.0 x 150 mm Shimadzu VP-ODS column. Eight flavonoids were identified to exist in Astragali Radix based on their characteristic UV data and mass spectra. The concentrations of three major components in this herb--ononin, calycosin and formononetin--were determined by LC/ESI-MS in positive selective ion monitoring (SIM) mode. The calibration curves were linear in the range of 0.9~180.0 mug.mL(-)(1) for ononin, 1.8~360.0 mug.mL(-)(1) for calycosin and 1.4~280 mug.mL(-)(1) for formononetin, respectively. The limits of quantification (LOQ) and detection (LOD) were 0.9 mug. mL(-)(1) and 0.2 mug mL(-)(1) for ononin, 1.8 mug mL(-)(1) and 0.5 mug.mL-1 for calycosin, 1.4 mug mL(-)(1) and 0.5 mug.mL(-)(1) for formononetin, respectively. The standard recoveries were between 95.4~104.7%. The developed method was proven to be useful for the quantitative and qualitative analysis of flavonoid constituents in various resources of Astragali Radix. FAU - Lv, Yan-Wen AU - Lv YW AD - Department of Chemistry and Material Engineering, Quzhou College, Quzhou 324000, China. FAU - Hu, Wei AU - Hu W FAU - Wang, Yu-Ling AU - Wang YL FAU - Huang, Lan-Fang AU - Huang LF FAU - He, Yun-Biao AU - He YB FAU - Xie, Xian-Zhen AU - Xie XZ LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20110309 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Flavonoids) RN - 0 (Plant Extracts) SB - IM MH - Astragalus Plant/*chemistry MH - Chromatography, High Pressure Liquid/*methods MH - Chromatography, Liquid MH - Flavonoids/*analysis MH - Plant Extracts/*analysis MH - Spectrometry, Mass, Electrospray Ionization/*methods PMC - PMC6259829 EDAT- 2011/03/11 06:00 MHDA- 2011/06/17 06:00 PMCR- 2011/03/09 CRDT- 2011/03/11 06:00 PHST- 2011/01/10 00:00 [received] PHST- 2011/02/24 00:00 [revised] PHST- 2011/03/04 00:00 [accepted] PHST- 2011/03/11 06:00 [entrez] PHST- 2011/03/11 06:00 [pubmed] PHST- 2011/06/17 06:00 [medline] PHST- 2011/03/09 00:00 [pmc-release] AID - molecules16032293 [pii] AID - molecules-16-02293 [pii] AID - 10.3390/molecules16032293 [doi] PST - epublish SO - Molecules. 2011 Mar 9;16(3):2293-303. doi: 10.3390/molecules16032293.