PMID- 2139101
OWN - NLM
STAT- MEDLINE
DCOM- 19900517
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 171
IP  - 4
DP  - 1990 Apr 1
TI  - The glycosyl phosphatidylinositol-linked Fc gamma RIIIPMN mediates transmembrane 
      signaling events distinct from Fc gamma RII.
PG  - 1239-55
AB  - To investigate the ability of FcgammaRIII(PMN), the GPI-anchored isoform of 
      FcgammaRIII (CD16) in polymorphonuclear leukocytes (PMN), to mediate 
      transmembrane signaling events, we measured changes in membrane potential with 
      DiOC(5) and in intracellular calcium with indo-1. FcgammaR were ligated by 
      anti-FcgammaRIII mAb 3G8 (IgG and Fab), anti-FcgammaRII mAb IV.3 (IgG and Fab), 
      and human IgG aggregates. Cell bound mAbs were also crosslinked by goat F(ab')(2) 
      anti-mouse IgG. 3G8 IgG elicited a rapid change in [Ca(2+)](i), which was 
      unaffected by EGTA, Vibrio cholerae toxin (CT), or Bordetella pertussis toxin 
      (PT), and was abolished by BAPTA . Univalent receptor binding with 3G8 Fab gave 
      no response but crosslinking with F(aV)2 GAM gave a rapid [Ca2,](i) response. 
      Neither IV.3 Fab, IV.3 IgG, nor crosslinking of IV.3 Fab elicited a calcium 
      signal. PI-PLC-treated PMN with the density of FcgammaRIII(PMN) reduced to that 
      of FcgammaRII showed an unattenuated change in [Ca(2+)](i), with a 3G8 stimulus. 
      The effects of IgG aggregates paralleled those of 3G8 mAb. These data indicate 
      that multivalent ligation of FcgammaRIII(PMN) initiates an increase in [Ca(2+)];, 
      derived from intracellular stores, that is distinct from both the FMLP- and 
      FcgammaRII-induced responses. Ligand-dependent interaction with FcgammaRII is not 
      required. Since FcgammaRIII(PMN) can internalize the FcgammaRIII-specific probe 
      Con A-opsonized E and lyse anti-FcgammaRIII heteroantibody-opsonized chick E, 
      this GPI-anchored molecule mediates both signal transduction and integrated cell 
      responses.
FAU - Kimberly, R P
AU  - Kimberly RP
AD  - Hospital for Special Surgery, Cornell University Medical College, New York, New 
      York 10021.
FAU - Ahlstrom, J W
AU  - Ahlstrom JW
FAU - Click, M E
AU  - Click ME
FAU - Edberg, J C
AU  - Edberg JC
LA  - eng
GR  - P60-AR38520/AR/NIAMS NIH HHS/United States
GR  - R01-AR33062/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Glycolipids)
RN  - 0 (Glycosylphosphatidylinositols)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Phosphatidylinositols)
RN  - 0 (Receptors, Fc)
RN  - 0 (Receptors, IgG)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Antigens, Differentiation/*physiology
MH  - Calcium/blood
MH  - Cell Membrane/drug effects/immunology/physiology
MH  - Chemotaxis, Leukocyte
MH  - Fluorescent Dyes
MH  - Glycolipids/*physiology
MH  - Glycosylphosphatidylinositols
MH  - Humans
MH  - Immunoglobulin G/metabolism
MH  - In Vitro Techniques
MH  - Kinetics
MH  - Membrane Potentials/drug effects
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/immunology/*physiology
MH  - Phosphatidylinositols/*physiology
MH  - Receptors, Fc/*physiology
MH  - Receptors, IgG
MH  - *Signal Transduction
MH  - Spectrometry, Fluorescence
PMC - PMC2187837
EDAT- 1990/04/01 00:00
MHDA- 1990/04/01 00:01
PMCR- 1990/10/01
CRDT- 1990/04/01 00:00
PHST- 1990/04/01 00:00 [pubmed]
PHST- 1990/04/01 00:01 [medline]
PHST- 1990/04/01 00:00 [entrez]
PHST- 1990/10/01 00:00 [pmc-release]
AID - 17141239 [pii]
AID - 10.1084/jem.171.4.1239 [doi]
PST - ppublish
SO  - J Exp Med. 1990 Apr 1;171(4):1239-55. doi: 10.1084/jem.171.4.1239.