PMID- 21391046
OWN - NLM
STAT- MEDLINE
DCOM- 20110617
LR  - 20120514
IS  - 1532-4230 (Electronic)
IS  - 1532-1819 (Linking)
VI  - 32
IP  - 2
DP  - 2011
TI  - Comparison of speedy PCR-ssp method and serological typing of HLA-A24 for 
      Japanese cancer patients.
PG  - 93-102
LID - 10.1080/15321819.2010.543219 [doi]
AB  - Human leukocyte antigen (HLA) typing is essential to carry out HLA-class I 
      restricted antigenic peptide-based cancer immunotherapy. To establish a one-step 
      polymerase chain reaction-sequence-specific primer (PCR-SSP) method, we designed 
      two novel HLA-A24-specific primer sets and determined the optimal conditions for 
      specific amplification. Then, we performed HLA-A24 typing of two healthy donors' 
      and 17 cancer patients' peripheral blood with serological typing and PCR-SSP 
      typing. Eleven of the 19 cases were determined HLA-A24-positive by the PCR-SSP 
      method precisely; however, five cases showed false positive with serological 
      analysis. Thus, for HLA-A24 typing in the Japanese population, the PCR-SSP method 
      is faster and more accurate than serological typing.
FAU - Nakatsugawa, Munehide
AU  - Nakatsugawa M
AD  - Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
FAU - Hirohashi, Yoshihiko
AU  - Hirohashi Y
FAU - Torigoe, Toshihiko
AU  - Torigoe T
FAU - Inoda, Satoko
AU  - Inoda S
FAU - Kiriyama, Kenji
AU  - Kiriyama K
FAU - Tamura, Yasuaki
AU  - Tamura Y
FAU - Sato, Eiji
AU  - Sato E
FAU - Takahashi, Hiroki
AU  - Takahashi H
FAU - Sato, Noriyuki
AU  - Sato N
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Immunoassay Immunochem
JT  - Journal of immunoassay & immunochemistry
JID - 100963688
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (DNA Primers)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A24 Antigen)
SB  - IM
MH  - Antibodies, Monoclonal/immunology
MH  - Base Sequence
MH  - DNA Primers
MH  - Female
MH  - Flow Cytometry
MH  - HLA-A Antigens/*genetics
MH  - HLA-A24 Antigen
MH  - Humans
MH  - Japan
MH  - Male
MH  - Neoplasms/*immunology
MH  - Polymerase Chain Reaction/*methods
MH  - Sequence Homology, Nucleic Acid
EDAT- 2011/03/11 06:00
MHDA- 2011/06/18 06:00
CRDT- 2011/03/11 06:00
PHST- 2011/03/11 06:00 [entrez]
PHST- 2011/03/11 06:00 [pubmed]
PHST- 2011/06/18 06:00 [medline]
AID - 934608426 [pii]
AID - 10.1080/15321819.2010.543219 [doi]
PST - ppublish
SO  - J Immunoassay Immunochem. 2011;32(2):93-102. doi: 10.1080/15321819.2010.543219.