PMID- 21393878
OWN - NLM
STAT- MEDLINE
DCOM- 20110620
LR  - 20110311
IS  - 0974-5130 (Electronic)
IS  - 0377-4929 (Linking)
VI  - 54
IP  - 1
DP  - 2011 Jan-Mar
TI  - Light chain immunofluorescence in various nephropathies.
PG  - 55-8
LID - 10.4103/0377-4929.77325 [doi]
AB  - CONTEXT: Light chain immunofluoresence (IF) in renal biopsy is routinely used in 
      the diagnosis of light chain deposition disease (LCDD), amyloidosis and cast 
      nephropathy. Light chain predominance has also been reported in certain 
      glomerulopathies like IgA nephropathy. However, pathogenesis of this pattern of 
      deposition in various glomerulopathies is uncertain. AIM: To discuss the 
      pathogenesis and utility of light chain IF in nephropathies. SETTING AND DESIGN: 
      Retrospective study. MATERIALS AND METHODS: The pattern of light chain IF and 
      light microscopic diagnosis in 306 cases of various nephropathies was reviewed. 
      Direct IF was done in all these cases with commercial fluorescence (Fluoresciene 
      Isothiocynate ) conjugated polyclonal rabbit anti-human antisera against IgM, 
      IgG, IgA, C3, C1q, kappa and lambda light chains. RESULTS: Light chain deposits 
      were seen in 240 (78.43%) cases. In IgA nephropathy, lupus nephritis and 
      post-infectious glomerulonephritis (PIGN), lambda positivity was more as compared 
      to kappa. Light chain deposits in LCDD and membranous nephropathy were more kappa 
      type. The IF pattern in amyloidosis was not consistent. CONCLUSION: The 
      pathogenesis of light chain predominance in glomerulopathies is not clear and it 
      depends on isoelectric point and size of the immune complex. Light chain IF 
      should be performed routinely in all the renal biopsies.
FAU - Uppin, Megha S
AU  - Uppin MS
AD  - Department of Pathology, Nizam's Institute of Medical Sciences, Hyderabad 500 
      082, Andhra Pradesh, India. megha_harke@yahoo.co.in
FAU - Prayaga, Aruna K
AU  - Prayaga AK
FAU - Srinivas, B H
AU  - Srinivas BH
FAU - Rapur, Ram
AU  - Rapur R
FAU - Desai, Madhav
AU  - Desai M
FAU - Dakshina Murthy, K V
AU  - Dakshina Murthy KV
LA  - eng
PT  - Journal Article
PL  - India
TA  - Indian J Pathol Microbiol
JT  - Indian journal of pathology & microbiology
JID - 7605904
RN  - 0 (Complement C3)
RN  - 0 (Immunoglobulin Light Chains)
RN  - 80295-33-6 (Complement C1q)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Child
MH  - Child, Preschool
MH  - Complement C1q/analysis
MH  - Complement C3/analysis
MH  - Female
MH  - *Fluorescent Antibody Technique
MH  - Humans
MH  - Immunoglobulin Light Chains/*analysis
MH  - Infant
MH  - Kidney Diseases/*pathology
MH  - Male
MH  - Middle Aged
MH  - Retrospective Studies
MH  - Young Adult
EDAT- 2011/03/12 06:00
MHDA- 2011/06/21 06:00
CRDT- 2011/03/12 06:00
PHST- 2011/03/12 06:00 [entrez]
PHST- 2011/03/12 06:00 [pubmed]
PHST- 2011/06/21 06:00 [medline]
AID - IndianJPatholMicrobiol_2011_54_1_55_77325 [pii]
AID - 10.4103/0377-4929.77325 [doi]
PST - ppublish
SO  - Indian J Pathol Microbiol. 2011 Jan-Mar;54(1):55-8. doi: 10.4103/0377-4929.77325.