PMID- 21396175
OWN - NLM
STAT- MEDLINE
DCOM- 20120308
LR  - 20181201
IS  - 1555-3892 (Electronic)
IS  - 0963-6897 (Linking)
VI  - 20
IP  - 9
DP  - 2011
TI  - Human umbilical cord mesenchymal stem cells ameliorate mice trinitrobenzene 
      sulfonic acid (TNBS)-induced colitis.
PG  - 1395-408
LID - 10.3727/096368910X557245 [doi]
AB  - Mesenchymal stem cells (MSCs), which are poorly immunogenic and have potent 
      immunosuppressive activities, have emerged as a promising candidate for cellular 
      therapeutics for the treatment of disorders caused by abnormal immune responses. 
      In this study we investigated whether human umbilical cord-derived mesenchymal 
      stem cells (hUC-MSCs) could ameliorate colitis in a trinitrobenzene sulfonic acid 
      (TNBS)-induced colitis model. TNBS-treated colitic mice were infused with 
      hUC-MSCs or vehicle control. The mice were sacrificed on day 1, 3, and 5 after 
      infusion, and their clinical and pathological conditions were evaluated by body 
      weight, colon length, and histological analysis. The expression levels of 
      proinflammatory cytokine proteins in colon were examined by ELISA. The homing of 
      hUC-MSCs was studied by live in vivo imaging and immunofluorescent microscopy. 
      hUC-MSCs were found to migrate to the inflamed colon and effectively treated the 
      colitic mice with improved clinical and pathological signs. The levels of IL-17 
      and IL-23 as well as IFN-gamma and IL-6 were significantly lower in the colon tissues 
      of the hUC-MSC-treated mice in comparison with the vehicle-treated mice. 
      Coculture experiments showed that hUC-MSCs not only could inhibit IFN-gamma 
      expression but also significantly inhibit IL-17 production by lamina propria 
      mononuclear cells (LPMCs) or splenocytes of the colitic mice or by those isolated 
      from normal animals and stimulated with IL-23. Systemically infused hUC-MSCs 
      could home to the inflamed colon and effectively ameliorate colitis. In addition 
      to the known suppressive effects on Th1-type immune responses, hUC-MSC-mediated 
      modulation of IL-23/IL-17 regulated inflammatory reactions also plays an 
      important role in the amelioration of colitis.
FAU - Liang, Lu
AU  - Liang L
AD  - State Key Laboratory of Experimental Hematology, Institute of Hematology & 
      Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union 
      Medical College, Tianjin, PR China.
FAU - Dong, Chunlan
AU  - Dong C
FAU - Chen, Xiaojun
AU  - Chen X
FAU - Fang, Zhihong
AU  - Fang Z
FAU - Xu, Jie
AU  - Xu J
FAU - Liu, Meng
AU  - Liu M
FAU - Zhang, Xiaoguang
AU  - Zhang X
FAU - Gu, Dong Sheng
AU  - Gu DS
FAU - Wang, Ding
AU  - Wang D
FAU - Du, Weiting
AU  - Du W
FAU - Zhu, Delin
AU  - Zhu D
FAU - Han, Zhong Chao
AU  - Han ZC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110309
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 8T3HQG2ZC4 (Trinitrobenzenesulfonic Acid)
SB  - IM
MH  - Animals
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Cell Separation
MH  - Cell Survival
MH  - Coculture Techniques
MH  - Colitis/*pathology/*therapy
MH  - Colon/pathology
MH  - Cytokines/biosynthesis
MH  - Humans
MH  - Immunophenotyping
MH  - Inflammation Mediators/metabolism
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Microscopy, Fluorescence
MH  - Mucous Membrane/pathology
MH  - Spleen/metabolism/pathology
MH  - Trinitrobenzenesulfonic Acid
MH  - Umbilical Cord/*cytology
EDAT- 2011/03/15 06:00
MHDA- 2012/03/09 06:00
CRDT- 2011/03/15 06:00
PHST- 2011/03/15 06:00 [entrez]
PHST- 2011/03/15 06:00 [pubmed]
PHST- 2012/03/09 06:00 [medline]
AID - ct2229liang [pii]
AID - 10.3727/096368910X557245 [doi]
PST - ppublish
SO  - Cell Transplant. 2011;20(9):1395-408. doi: 10.3727/096368910X557245. Epub 2011 
      Mar 9.