PMID- 21396709
OWN - NLM
STAT- MEDLINE
DCOM- 20110726
LR  - 20220316
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 32
IP  - 15
DP  - 2011 May
TI  - The influence of stereolithographic scaffold architecture and composition on 
      osteogenic signal expression with rat bone marrow stromal cells.
PG  - 3750-63
LID - 10.1016/j.biomaterials.2011.01.016 [doi]
AB  - Scaffold design parameters, especially physical construction factors such as 
      mechanical stiffness of substrate materials, pore size of 3D porous scaffolds, 
      and channel geometry, are known to influence the osteogenic signal expression and 
      subsequent differentiation of a transplanted cell population. In this study of 
      photocrosslinked poly(propylene fumarate) (PPF) and diethyl fumarate (DEF) 
      scaffolds, the effect of DEF incorporation ratio and pore size on the osteogenic 
      signal expression of rat bone marrow stromal cells (BMSCs) was investigated. 
      Results demonstrated that DEF concentrations and pore sizes that led to increased 
      scaffold mechanical stiffness also upregulated osteogenic signal expression, 
      including bone morphogenic protein-2 (BMP-2), fibroblast growth factors-2 
      (FGF-2), transforming growth factor-beta1 (TGF-beta1), vascular endothelial growth 
      factor (VEGF), and Runx2 transcriptional factor. Similar scaffold fabrication 
      parameters supported rapid BMSC osteoblastic differentiation, as demonstrated by 
      increased alkaline phosphatase (ALP) and osteocalcin expression. When scaffolds 
      with random architecture, fabricated by porogen leaching, were compared to those 
      with controlled architecture, fabricated by stereolithography (SLA), results 
      showed that SLA scaffolds with the highly permeable and porous channels also have 
      significantly higher expression of FGF-2, TGF-beta1, and VEGF. Subsequent ALP 
      expression and osteopontin secretion were also significantly increased in SLA 
      scaffolds. Based upon these results, we conclude that scaffold properties 
      provided by additive manufacturing techniques such as SLA fabrication, 
      particularly increased mechanical stiffness and high permeability, may stimulate 
      dramatic BMSC responses that promote rapid bone tissue regeneration.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Kim, Kyobum
AU  - Kim K
AD  - Department of Chemical and Biomolecular Engineering, University of Maryland, 3238 
      Jeong H. Kim Engineering Building, College Park, MD 20742, United States.
FAU - Dean, David
AU  - Dean D
FAU - Wallace, Jonathan
AU  - Wallace J
FAU - Breithaupt, Rob
AU  - Breithaupt R
FAU - Mikos, Antonios G
AU  - Mikos AG
FAU - Fisher, John P
AU  - Fisher JP
LA  - eng
GR  - R01 DE013740/DE/NIDCR NIH HHS/United States
GR  - R01 DE013740-06A1/DE/NIDCR NIH HHS/United States
GR  - R01-DE013740/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Fumarates)
RN  - 0 (Polypropylenes)
RN  - 0 (poly(propylene fumarate))
RN  - 5WBU5A3E8A (diethyl fumarate)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology/metabolism
MH  - Cells, Cultured
MH  - Fumarates/chemistry
MH  - Male
MH  - *Osteogenesis
MH  - Polypropylenes/chemistry
MH  - Porosity
MH  - Rats
MH  - Rats, Wistar
MH  - Stromal Cells/*cytology/metabolism
MH  - Tissue Scaffolds/*chemistry
PMC - PMC3075725
MID - NIHMS275542
EDAT- 2011/03/15 06:00
MHDA- 2011/07/27 06:00
PMCR- 2012/05/01
CRDT- 2011/03/15 06:00
PHST- 2010/12/21 00:00 [received]
PHST- 2011/01/06 00:00 [accepted]
PHST- 2011/03/15 06:00 [entrez]
PHST- 2011/03/15 06:00 [pubmed]
PHST- 2011/07/27 06:00 [medline]
PHST- 2012/05/01 00:00 [pmc-release]
AID - S0142-9612(11)00029-9 [pii]
AID - 10.1016/j.biomaterials.2011.01.016 [doi]
PST - ppublish
SO  - Biomaterials. 2011 May;32(15):3750-63. doi: 10.1016/j.biomaterials.2011.01.016.